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This Article Accepted manuscript (PDF) All Versions of this Article: JME-08-0068v1 42/2/139    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Bernabucci, U. Articles by Nardone, A. Search for Related Content PubMed PubMed Citation Articles by Bernabucci, U. Articles by Nardone, A.

U Bernabucci, Produzioni Animali, Università della Tuscia, Viterbo, 01100, Italy
L Basirico', Produzioni Animali, Universita' della Tuscia, viterbo, Italy
P Morera, viterbo, Italy
N Lacetera, viterbo, Italy
B Ronchi, viterbo, Italy
A Nardone, viterbo, Italy

Correspondence: Umberto Bernabucci, Email: bernab{at}unitus.it

Abstract

Studies have demonstrated that heat shock is associated with alteration in energy metabolism. In this study, we investigated the effect of heat shock on gene expression and secretion of adiponectin and leptin, and gene expression of HSP-70.2 and PPAR in 3T3-L1 adipocytes. Compared with 37 °C, adiponectin mRNA was higher at 39 °C, and lower at 41 °C. Leptin mRNA was higher when adipocytes were exposed to 41 °C compared with 37 and 39 °C. Secretion of adiponectin increased at 39 °C, and when cells were exposed to 41 °C it was not detectable. Leptin secretion increased significantly at 41 °C, compared with 37 and 39 °C. Hsp-70.2 mRNA was increased at 39 °C, and the highest level was reached at 41 °C. PPAR mRNA exhibited a substantial increase in a temperature-dependent manner. The study provides the first evidence of a direct effect of heat shock on adiponectin and leptin gene expression and secretion, and demonstrates that the expression of the two adipokines is differentially regulated at the temperatures tested.