HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Makridakis, N. Articles by Reichardt, J. K V Search for Related Content PubMed PubMed Citation Articles by Makridakis, N. Articles by Reichardt, J. K V

¹ Department of Biochemistry and Molecular Biology and Institute for Genetic Medicine, Keck School of Medicine of the University of Southern California, 2250 Alcazar Street, IGM240, Los Angeles, California 90089-9075, USA
² Department of Preventive Medicine, Keck School of Medicine of the University of Southern California, 2250 Alcazar Street, IGM240, Los Angeles, California 90089-9075, USA

(Requests for offprints should be addressed to J K V Reichardt, 2250 Alcazar Street, IGM240, Los Angeles, CA 90089-9075, USA; Email: reichard{at}usc.edu)

(J K V Reichardt is currently at University of Sydney, Medical Foundation Building (K25), 92-94 Parramatta Rd, Camperdown, New South Wales 2042, Australia)

	Abstract

Top Abstract Introduction Materials and methods Results Discussion References

 
Human steroid 5-reductase type II is a prostate-specific, membrane-associated enzyme that catalyzes the conversion of testosterone to dihydrotestosterone, the most potent androgen in the prostate gland. Genetic variants of this enzyme have been associated with both the development and the progression of prostate cancer. Both finasteride and dutasteride are competitive inhibitors of the type II steroid 5-reductase that have been effectively used for the treatment of benign prostatic hyperplasia. Finasteride has also been successfully utilized for prostate cancer chemoprevention. We here investigate 5-reductase inhibition assays in vitro to measure the effect of incubation time on the apparent inhibition constant (K_i) for both constitutional and somatic (prostate cancer) enzyme variants. Our systematic pharmacogenetic analysis shows that both finasteride and dutasteride are slow, time-dependent inhibitors of steroid 5-reductase type II, and that the inhibition kinetics depend on the 5-reductase genotype. We also show that, overall, dutasteride is a more efficient steroid 5-reductase inhibitor than finasteride. Based on our data, we are able to map areas of the enzyme that are responsible for this time-dependent inhibition for either (or both) enzyme inhibitor(s). This comprehensive pharmacogenetic analysis of steroid 5-reductase variants unveiled significant pharmacogenetic variation for both finasteride and dutasteride and thus should be taken into account when designing protocols for treatment and/or chemoprevention of prostatic diseases with either one of these 5-reductase inhibitors since there is considerable pharmacogenetic variation for both drugs.

	Introduction

Top Abstract Introduction Materials and methods Results Discussion References

 
The prostate gland depends on androgens for both its growth and development (Cheng et al. 1993). We have reported evidence that increased intraprostatic androgen metabolism, particularly through genetic variants of the enzyme steroid 5-reductase, may play an important role in both predisposition to prostate cancer (Makridakis et al. 1999) as well as prostate cancer progression (Makridakis et al. 2004). Human prostatic (type II) steroid 5-reductase is encoded by the SRD5A2 gene located in chromosome band 2p23 (Russell & Wilson 1994). Steroid 5-reductase is a membrane-associated enzyme that catalyzes the conversion of testosterone to dihydrotestosterone (DHT), the most potent androgen in the prostate (e.g. Cheng et al. 1993). Thus, genetic variants encoded by the SRD5A2 gene may play an important role in both the development and the progression of prostate cancer (Ross et al. 1998).

Finasteride is a specific competitive inhibitor of the prostatic (type II) steroid 5-reductase, both in vivo and in vitro (Stoner 1996, Span et al. 1999). These properties have made finasteride an attractive drug for the treatment of benign prostatic hyperplasia (BPH) (McConnell et al. 1998, Lowe et al. 2003) and prostate cancer chemoprevention (Thompson et al. 2003), with successful results. Specifically, the prostate cancer prevention trial (PCPT) showed that treatment of men 55 years of age or older with finasteride resulted in a significant decrease in prostate cancer incidence measured over a seven-year period (Thompson et al. 2003), suggesting that finasteride prevents or delays the appearance of prostate cancer. However, finasteride treatment also resulted in a significant increase in the incidence of prostate tumors of high grade (7 or higher) Gleason score (Thompson et al. 2003). Dutasteride (or GG745), a competitive inhibitor of both steroid 5-reductase isozymes (type I and type II; Bramson et al. 1997), has also been shown to improve the symptoms associated with BPH without any significant increase in adverse side effects (Brown & Nuttall 2003, Clark et al. 2004).

We have reported significant genetic and pharmacogenetic variation for both finasteride and dutasteride at the SRD5A2 locus in both somatic prostate cancer tissue and constitutional DNA of prostate cancer patients (Makridakis et al. 2000, 2004). Some of these SRD5A2 mutations resulted in increased enzyme activity and lowered inhibition by finasteride (Makridakis et al. 2000, 2004). These findings suggest that the presence of specific SRD5A2 mutants that are not efficiently inhibited by finasteride may, at least partially, explain the unexpected finding of an increase in high grade prostate cancer rate in the PCPT (reported by Thompson et al. 2003) since these mutants might result in more aggressive growth of tumors.

The kinetics of steroid 5-reductase inhibition that we reported previously (Makridakis et al. 2000, 2004) were based on 10-min incubations for all enzyme variants. Finasteride, however, dissociates from the enzyme very slowly, and therefore is a time-dependent inhibitor of steroid 5-reductase (Faller et al. 1993, Tian et al. 1994, 1995). In addition, dutasteride (Frye et al. 1998), like finasteride, retains the ^1,2 bond that is critical for time-dependent inhibition in 4-azasteroids (Russell & Wilson 1994). Thus we decided to compare the kinetics of steroid 5-reductase inhibition using 10- and 30-min incubation of the enzyme with either finasteride or dutasteride.

We report here that, like finasteride, dutasteride is a time-dependent inhibitor of steroid 5-reductase type II. We also show that dutasteride is a more efficient steroid 5-reductase inhibitor than finasteride for most of the enzyme variants that we studied. Based on our data, we are able to map areas of the enzyme that when mutated result in time-independent inhibition for either (or both) enzyme inhibitor(s). This comprehensive pharmacogenetic analysis of steroid 5-reductase variants unveiled significant pharmacogenetic variation for both finasteride and dutasteride and this should be taken into account when designing protocols for treatment and/or chemoprevention of prostatic diseases with either one of these drugs.

	Materials and methods

Top Abstract Introduction Materials and methods Results Discussion References

 
Steroid inhibitors

Finasteride was obtained from Merck (Rahway, NJ, USA) and dutasteride was synthesized by Pharmacia & Upjohn (Nerviano, Italy) (Makridakis et al. 2000).

Pharmacogenetic analysis

Individual missense substitutions were reconstructed in the SRD5A2 cDNA mammalian expression vector pS303–5R2 (originally obtained from Dr D Russell, UT Southwestern Medical College, Dallas, TX, USA), by site-directed mutagenesis using the QuickChange kit (Stratagene, San Diego, CA, USA) with custom primers (Invitrogen, Carlsbad, CA, USA) (Makridakis et al. 2000). The various SRD5A2 cDNAs were electroporated into COS 7 cells together with a ß-galactosidase control plasmid (pCMVß, obtained originally from Dr Larry Kedes, University of Southern California, Los Angeles, CA, USA), and the respective protein extracts were collected 48 h later, by sonication. Total protein was quantified with a BioRad protein assay (Hercules, CA, USA) and ß-galactosidase assay was then performed to normalize the extracts for differences in transfection efficiency (Makridakis et al. 2000). Normalized extract amounts were then used for 5-reductase activity assay, as described (Makridakis et al. 2000). Briefly, a mixture containing 5 mM NADPH (Sigma, St Louis, MO, USA) and normalized protein extracts was added to specific amounts of each inhibitor at room temperature. Then, 0.8 or 1.6 µM [¹⁴C]testosterone (NEN, Boston, MA, USA) and 0.1 M Tris–citrate buffer of optimum pH for each variant (Makridakis et al. 2000, 2004) were added to the mix, and the reactions were placed at 37 °C for either 10 or 30 min. Initially, the concentration of the inhibitor was varied in 10-fold increments between 10^–10 and 10^–5 M, to approximate the apparent inhibition constant (K_i). After the initial approximation, the concentration was varied in five increments around the approximate K_i in triplicate experiments. Following incubation at 37 °C, the reactions were stopped with methylene chloride, and after extraction of the organic phase, steroids were dried, redissolved in ethanol and separated by thin-layer chromatography (TLC) using K6 silica TLC plates (Whatman, Clifton, NJ, USA) (Makridakis et al. 1999, 2000). Dried TLC plates were then exposed on phosphorimager screens (Molecular Dynamics, Mountain View, CA, USA) and quantified on a Storm phosphorimager (Molecular Dynamics). Then, substrate-to-product conversion values were translated into velocity values and plotted using Cricket Graph 1.3 (Cricket Software, Malvern, PA, USA). Linear regression analysis of the respective Lineweaver-Burk plots was used to calculate K_i values. The apparent K_i for each variant was determined by averaging the K_i values from three independent experiments. Further experimental details can be found in Makridakis et al.(2000).

	Results

Top Abstract Introduction Materials and methods Results Discussion References

 
We previously reported significant genetic and pharmacogenetic variation at the SRD5A2 locus for both finasteride and dutasteride in both somatic prostate cancer tissue and constitutional DNA of prostate cancer patients (Makridakis et al. 2000, 2004). Some of the SRD5A2 mutations we characterized are common and/or recurrent, including the A49T (alanine-49 to threonine) mutation, which has been found to be involved in prostate cancer predisposition and progression (Makridakis et al. 1999, 2004). The apparent K_i for both finasteride and dutasteride in those experiments was calculated after transient expression of the reconstructed variant clones in mammalian cells followed by 10-min in vitro enzyme assays (Makridakis et al. 2000, 2004). However, finasteride is a slow, time-dependent inhibitor of steroid 5-reductase (Faller et al. 1993, Tian et al. 1994, 1995). Therefore, longer finasteride incubations with the wild-type enzyme are expected to result in better inhibition, or lower apparent K_i (see e.g. Moss et al. 1996). We decided to test this hypothesis for both finasteride and dutasteride and all the steroid 5-reductase type II variants, using 30-min incubations.

As expected, the apparent K_i for finasteride dropped significantly, from 60 nM for the 10-min enzyme assays to 23 nM for the 30-min enzyme assays for the normal (aka wild-type) protein (Table 1). The apparent K_i for dutasteride also dropped significantly, from 17 nM to 4.3 nM (Table 2), suggesting that, like finasteride, the 4-azasteroid dutasteride is also a slow, time-dependent inhibitor of steroid 5-reductase type II. The majority of the steroid 5-reductase enzyme variants resulted in a reduction of the apparent K_i for both inhibitors from the 10-min to the 30-min reaction, and thus behave like the wild-type enzyme (Tables 1 and 2). The significance of these findings is emphasized by the small variability observed in the values measured from three independent experiments (Tables 1 and 2 and data not shown). However, several variants resulted in an increase of the apparent K_i for each inhibitor or no significant change between the 10- and 30-min reactions (Fig. 1 and Tables 1 and 2), and thus they exhibit time-independent inhibition. The steroid 5-reductase variants that resulted in time-independent inhibition affect enzyme residues in both the amino-terminus and the carboxy-terminus of the protein (residues 49, 118, 226, 227 and 234; Fig. 1), and include the A49T, a gain-of-function mutation in the enzyme (Makridakis et al. 1999) that has been associated with both predisposition and progression of prostate cancer (Makridakis et al. 1999, Jaffe et al. 2000, Makridakis et al. 2004).

View larger version (6K): [in this window] [in a new window]   Figure 1 Mapping of enzyme residues that do not show time-dependent inhibition for finasteride (regular font) and dutasteride (italics). Black blocks under the sequence indicate 5-reductase enzyme binding regions for both finasteride and dutasteride (deduced from the effect of specific enzyme variants on the apparent Ki).

Overall, we report a significant pharmacogenetic variation for both somatic and constitutional SRD5A2 variants and for both finasteride and dutasteride (Tables 1 and 2). The distribution of the apparent K_i for finasteride for the distinct SRD5A2 missense substitutions varied 84-fold (5–420 nM) in the 10-min reactions and 142-fold (1.9–270 nM) in the 30-min reactions (Table 1). The distribution of the apparent K_i for dutasteride varied 85-fold (1.1- 93 nM) in the 10-min reactions and 30-fold (0.5–15 nM) in the 30-min reactions (Table 2). Thus, 30-min incubations with dutasteride resulted in the lowest apparent K_i values overall and the lowest variation (Tables 1 and 2).

	Discussion

Top Abstract Introduction Materials and methods Results Discussion References

 
Both finasteride and dutasteride are important 4-azasteroid inhibitors of steroid 5-reductase that have been successfully utilized in vivo for the treatment and prevention of prostatic diseases. We previously reported significant pharmacogenetic variation of steroid 5-reductase for both finasteride and dutasteride in both somatic prostate cancer tissue and constitutional DNA (Makridakis et al. 2000, 2004). Those results, however, were based on 10-min reactions, while finasteride is a slow, time-dependent inhibitor of steroid 5-reductase (e.g. Faller et al. 1993, Tian et al. 1994). Thus, we reasoned that longer incubations with either inhibitor would be better approximations of the true inhibition in vivo. Due to the lability of the enzyme in vitro and the loss of linearity in the DHT production versus time curve after 40 min for the overactive A49T mutant (data not shown), we decided to use 30-min incubations for all the enzyme variants as the longer reaction time.

Comparison of the apparent K_i values between our standard (10 min) reactions and the extended (30 min) reactions for finasteride (Table 1) indicates that, as reported previously, finasteride is a time-dependent inhibitor of steroid 5-reductase. This fact is not only true for the wild-type protein, but also for most of the enzyme variants, whether constitutional or somatic (Table 1). Dutasteride is a dual (i.e. type I and type II) 5-reductase inhibitor (Bramson et al. 1997) and thus may be more efficacious in reducing DHT levels in the blood, in vivo. In addition, most of the 5-reductase enzyme variants resulted in significantly lower apparent K_i values for dutasteride in the 30-min reactions compared with the 10-min reactions (Table 2). Therefore, dutasteride is, like finasteride, a slow, time-dependent inhibitor of steroid 5-reductase. This finding is not surprising since both dutasteride and finasteride retain the ^1,2 bond that is critical for time-dependent inhibition in 4-azasteroids (Russell & Wilson 1994). These results also suggest a similar mechanism of time-dependent steroid 5-reductase inhibition for both finasteride and dutasteride.

Comparison of the apparent K_i values for each inhibitor suggests that dutasteride was the steroid 5-reductase inhibitor that exhibited the lowest apparent K_i for most steroid 5-reductase variants, including the wild-type, in both the 10-min and the 30-min reactions (Tables 1 and 2). The only exceptions to this observation were the F194 L variant (independently of reaction time) and the P48R variant (but only in the 10-min reaction). The disease-relevant A49T variant is of particular interest, since it is inhibited much more efficiently by dutasteride than finasteride, irrespective of the reaction time (Tables 1 and 2). Overall, the dual 5-reductase inhibitor, dutasteride, has higher affinity for steroid 5-reductase type II than finasteride, irrespective of genotype. Dutasteride is also expected to result in lower pharmacogenetic variation than finasteride in vivo, since it displays significantly lower pharmacogenetic variation of the apparent K_i than finasteride in the 30-min assays (see below; Tables 1 and 2). Thus, this compound may also be a better choice in vivo.

Thirty-minute 5-reductase inhibition reactions are expected to be more representative of the in vivo 5-reductase inhibition than the 10-min reactions we previously reported (Makridakis et al. 2000, 2004). The distribution of the apparent K_i for the distinct SRD5A2 variants in the 30-min reactions varied 142-fold (1.9–270 nM) for finasteride (Table 1) and 30-fold (0.5 nM-15 nM) for dutasteride (Table 2). Thus the apparent K_i values for the distinct SRD5A2 variants vary widely, irrespective of reaction time. This significant SRD5A2 pharmacogenetic variation may be important in vivo and should be taken into account when using finasteride or dutasteride for the treatment or prevention of prostate cancer. The PCPT trial reported that finasteride treatment in men followed over seven years resulted in a significant decrease in the overall rates of prostate cancer, but also in a significant increase in the rates of high-grade prostate tumors (Thompson et al. 2003). This unexpected finding of an increase in high grade prostate cancer rate in the PCPT may, at least partially, be explained by the presence of specific SRD5A2 variants (like A49T) in a subset of the study population that are not efficiently inhibited by finasteride. For individuals carrying the A49T allele, dutasteride might have been a better choice (Table 2 versus Table 1). Thus, future trials such as the PCPT and treatment protocols using 5-reductase inhibitors should consider genotyping men for SRD5A2 variants. In fact, we note that the pharmacogenetic inhibition increases for finasteride with time (Table 1) while it decreases for dutasteride (Table 2). This may suggest that dutasteride has a ‘tighter’ range for allelic and somatic variants, which may lessen the still considerable impact of pharmacogenetic variation in vitro. Thus, this compound may be generally better tolerated in vivo as well.

The inclusion of a high number of naturally occurring constitutional or somatic (prostate cancer) SRD5A2 variants in our pharmacogenetic analysis allowed us to identify areas of the protein that are important for the slow, time-dependent inhibition, for both 4-azasteroid inhibitors (Fig. 1). The wild-type as well as most of the steroid 5-reductase variants resulted in a reduction of the apparent K_i for both inhibitors from the 10-min to the 30-min reaction, and thus display time-dependent inhibition (Tables 1 and 2). However, several 5-reductase variants resulted in time-independent inhibition: (a) the A49T and F118 L variants for finasteride, and (b) the A49T, L226P, R227Q and F234 L variants for dutasteride (Fig. 1, Tables 1 and 2). These enzyme variants affect residues in both the amino-terminus and the carboxy-terminus of the protein (Fig. 1). Comparison of the wild-type versus mutant residue at positions 49, 118, 226, 227 and 234 indicates that the mutant residues are less hydrophobic. Indeed, the average hydropathy index (Kyte & Doolittle 1982) for residues 49 and 118 (for finasteride) is +2.3 for the wild-type residues and +1.5 for the mutant residues. Similarly, for dutasteride, the average hydropathy index (Kyte & Doolittle 1982) for residues 49, 226, 227 and 234 is +1.0 for the wild-type residues and +0.3 for the mutant residues. Thus, hydrophobic interactions between the 4-azasteroid (finasteride/dutasteride) and specific wild-type 5-reductase residues may be important for the slow, time-dependent inhibition displayed by these inhibitors. Pharmacogenetic analysis of these 5-reductase variants with another 4-azasteroid, such as PNU157706 (di Salle et al. 1998) may be instrumental in confirming this observation.

In summary, our systematic analysis of both constitutional and somatic (prostate cancer) variants of steroid 5-reductase type II indicates that dutasteride is a more efficient steroid 5-reductase type II inhibitor than finasteride in vitro for most of the enzyme variants, and that dutasteride treatment is also expected to result in lower pharmacogenetic variation in vivo than finasteride treatment. However, the pharmacogenetic variation we uncovered for both finasteride and dutasteride is still very significant and this should be taken into account when designing protocols for treatment and/or chemoprevention of prostatic diseases with either one of these drugs. Our data also suggest that both finasteride and dutasteride are slow, time-dependent inhibitors of steroid 5-reductase type II and allow us to map areas of the wild-type enzyme that are (at least partially) responsible for this time-dependent inhibition for either (or both) enzyme inhibitor(s). Pharmacogenetic analyses such as the one presented here may one day help in achieving individualized prostate cancer treatment (and perhaps even prevention).

	Acknowledgements

 
This work was supported in part by grants from GlaxoSmithKline, NCIR0168581 and NCIPOI108964 (project 1) to J K V R. We thank Roger Rittmaster (GlaxoSmithKline) for helpful comments on this manuscript.

	References

Top Abstract Introduction Materials and methods Results Discussion References

 
Bramson HN, Hermann D, Batchelor KW, Lee FW, James MK & Frye SV 1997 Unique preclinical characteristics of GG745, a potent dual inhibitor of 5AR. Journal of Pharmacology and Experimental Therapeutics 282 1496–1502.[Abstract/Free Full Text]

Brown CT & Nuttall MC 2003 Dutasteride: a new 5-alpha reductase inhibitor for men with lower urinary tract symptoms secondary to benign prostatic hyperplasia. International Journal of Clinical Practice 57 705–709.[Web of Science][Medline]

Cheng E, Lee C & Grayhack J 1993 Endocrinology of the prostate. In Prostate Diseases, pp 57–71. Eds H Lepor & RK Lawson. Philadelphia, PA: WB Saunders.

Clark RV, Hermann DJ, Cunningham GR, Wilson TH, Morrill BB & Hobbs S 2004 Marked suppression of dihydrotestosterone in men with benign prostatic hyperplasia by dutasteride, a dual 5 alpha-reductase inhibitor. Journal of Clinical Endocrinology and Metabolism 89 2179–2184.[Abstract/Free Full Text]

Faller B, Farley D & Nick H 1993 Finasteride: a slow-binding 5 alpha-reductase inhibitor. Biochemistry 32 5705–5710.[CrossRef][Medline]

Frye SV, Bramson HN, Herman DJ, Lee FW, Sinhababu AK & Tian G 1998 Discovery and development of GG745, a potent inhibitor of both isozymes of 5 alpha-reductase. Pharmaceutical Biotechnology 11 393–422.[Medline]

Jaffe JM, Malkowicz SB, Walker SH, MacBride S, Peschel R, Tomaszewski J, van Arsdalen K, Wein AJ & Rebbeck TR 2000 Association of SRD5A2 genotype and pathological characteristics of prostate tumors. Cancer Research 60 1626–1630.[Abstract/Free Full Text]

Kyte J & Doolittle RF 1982 A simple method for displaying the hydropathic character of a protein. Journal of Molecular Biology 157 105–132.[CrossRef][Web of Science][Medline]

Lowe FC, McConnell JD, Hudson PB, Romas NA, Boake R, Lieber M, Elhilali M, Geller J, Imperto-McGinely J, Andriole GL, Bruskewitz RC, Walsh PC, Bartsch G, Nacey JN, Shah S, Pappas F, Ko A, Cook T, Stoner E, Waldstreicher J. The Finasteride Study Group (2003) Long-term 6-year experience with finasteride in patients with benign prostatic hyperplasia. Urology 61 791–796.[CrossRef][Medline]

McConnell JD, Bruskewitz R, Walsh P, Andriole G, Lieber M, Holtgrewe HL, Albertsen P, Roehrborn CG, Nickel JC, Wang DZ, Taylor AM & Waldstreicher J 1998 The effect of finasteride on the risk of acute urinary retention and the need for surgical treatment among men with benign prostatic hyperplasia. Finasteride Long-Term Efficacy and Safety Study Group. New England Journal of Medicine 338 557–563.[Abstract/Free Full Text]

Makridakis NM, Ross RK, Pike MC, Crocitto LE, Kolonel LN, Pearce CL, Henderson BE & Reichardt JKV 1999 A missense substitution in the SRD5A2 gene is associated with prostate cancer in African-American and Hispanic men in Los Angeles. Lancet 354 975–978.[CrossRef][Web of Science][Medline]

Makridakis NM, di Salle E & Reichardt JKV 2000 Biochemical and pharmacogenetic dissection of steroid 5-reductase type II. Pharmacogenetics 10 407–413.[CrossRef][Medline]

Makridakis NM, Akalu A & Reichardt JKV 2004 Identification and characterization of somatic steroid 5-reductase (SRD5A2) mutations in human prostate cancer tissue. Oncogene 23 7399–7405.[CrossRef][Medline]

Moss ML, Kuzmic P, Stuart JD, Tian G, Peranteau AG, Frye SV, Kadwell SH, Kost TA, Overton LK & Patel IR 1996 Inhibition of human steroid 5 alpha reductases types I and II by 6-aza-steroids: structural determinants of one-step vs two-step mechanism. Biochemistry 35 3457–3464.[Medline]

Ross RK, Pike MC, Coetzee GA, Reichardt JKV, Yu MC, Feigelson H, Stanczyk FZ, Kolonel LN & Henderson BE 1998 Androgen metabolism and prostate cancer: establishing a model of genetic susceptibility. Cancer Research 58 4497–4504.[Abstract/Free Full Text]

Russell DW & Wilson JD 1994 Steroid 5 alpha-reductase: two genes/two enzymes. Annual Review of Biochemistry 63 25–61.[Web of Science][Medline]

di Salle E, Giudici D, Radice A, Zaccheo T, Ornati G, Nesi M, Panzeri A, Delos S & Martin PM 1998 PNU 157706, a novel dual type I and II 5 alpha-reductase inhibitor. Journal of Steroid Biochemistry and Molecular Biology 64 179–186.[CrossRef][Web of Science][Medline]

Span PN, Voller MC, Smals AG, Sweep FG, Schalken JA, Feneley MR & Kirby RS 1999 Selectivity of finasteride as an in vivo inhibitor of 5 alpha-reductase isozyme enzymatic activity in the human prostate. Journal of Urology 161 332–337.[CrossRef][Medline]

Stoner E 1996 5-Reductase inhibitors/finasteride. Prostate 6 82–87.[CrossRef]

Thompson IM, Goodman PJ, Tangen CM, Lucia MS, Miller GJ, Ford LG, Lieber MM, Cespedes RD, Atkins JN, Lippman SM, Carlin SM, Ryan A, Szczepanek CM, Crowley JJ & Coltman CA Jr 2003 The influence of finasteride on the development of prostate cancer. New England Journal of Medicine 349 215–224.[Abstract/Free Full Text]

Tian G, Stuart JD, Moss ML, Domanico PL, Bramson HN, Patel IR, Kadwell SH, Overton LK, Kost TA, Mook RA Jr, Frye SV, Batchelor KW & Wiseman JS 1994 17 Beta-(N-tert-butylcarbamoyl)-4-aza-5 alpha-androstan-1-en-3-one is an active site-directed slow time-dependent inhibitor of human steroid 5 alpha-reductase 1. Biochemistry 33 2291–2296.[CrossRef][Medline]

Tian G, Mook RA Jr, Moss ML & Frye SV 1995 Mechanism of time-dependent inhibition of 5 alpha-reductases by delta 1–4-azasteroids: toward perfection of rates of time-dependent inhibition by using ligand-binding energies. Biochemistry 34 13453–13459.[CrossRef][Medline]

Received 22 July 2004
Accepted 1 February 2005
Made available online as an Accepted Preprint 9 February 2005

This article has been cited by other articles:

				J. Li, R. J. Coates, M. Gwinn, and M. J. Khoury Steroid 5-{alpha}-Reductase Type 2 (SRD5a2) Gene Polymorphisms and Risk of Prostate Cancer: A HuGE Review Am. J. Epidemiol., January 1, 2010; 171(1): 1 - 13. [Abstract] [Full Text] [PDF]

This Article Abstract Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Makridakis, N. Articles by Reichardt, J. K V Search for Related Content PubMed PubMed Citation Articles by Makridakis, N. Articles by Reichardt, J. K V