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An isolated in-vitro perifused interrenal gland preparation from the dogfish Scyliorhinus canicula was used to study production of quantitatively the major corticosteroid 1-hydroxycorticosterone (1-OH-B), measured by radioimmunoassay. Basal secretory rates were 877·1 ± 145 (S.E.M.) fmol/mg per 15 min (n=14) and the preparation remained viable for up to 22 h, as reflected in a brisk response to 10 µM cyclic AMP (cAMP) after this time. Steroid production responded in a dose-dependent manner to porcine ACTH, with 10 µM producing a maximum stimulation of 225% above the basal secretory rate. cAMP (10 µM) produced an increase of 278% above basal, while 1 µM forskolin increased basal secretory rates by 127%. [Val⁵]- and [Ile⁵]-angiotensin II (0·1 µM) increased 1-OH-B production by 120 and 372% respectively over basal secretory rates. Increasing the concentration of K⁺ in the perfusate from 8 mm to 12, 18, 28 and 40 mM produced a significant rise only at 28 mM. Alterations in the concentration of Na⁺ and osmolarity of the perifusion medium had inconsistent effects on steroid production. Increased concentrations of urea (from 360 to 720 mM) increased the basal secretory rate by 121%, whilst reducing the concentration of urea (from 360 to 90 mM) had no effect.

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				S. M. Carroll, J. T. Bridgham, and J. W. Thornton Evolution of Hormone Signaling in Elasmobranchs by Exploitation of Promiscuous Receptors Mol. Biol. Evol., December 1, 2008; 25(12): 2643 - 2652. [Abstract] [Full Text] [PDF]