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This Article Full Text Full Text (PDF) All Versions of this Article: JME-09-0046v1 43/4/171    most recent Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Google Scholar Articles by Hoggard, N Articles by Miller, J D B PubMed PubMed Citation Articles by Hoggard, N Articles by Miller, J D B

Rowett Institute of Nutrition and Health, Aberdeen Centre for Energy Regulation and Obesity (ACERO), University of Aberdeen, Bucksburn, Aberdeen, Scotland AB21 9SB, UK
¹ Department of Surgery, Dr Gray's Hospital, Elgin, UK

(Correspondence should be addressed to N Hoggard; Email: nh{at}rowett.ac.uk)

Inhibin βB (INHBB; coding for the activin βB subunit) has previously been identified in both human and rodent adipose tissue and using Taqman real-time PCR with specific primers we confirm the expression of INHBB mRNA in rodent adipose tissue. Expression of INHBB in murine epididymal adipose tissue was higher than in any of the other tissues studied and appears to be regulated by changes in energy balance and leptin. It was increased fourfold in the epididymal fat depot of ob/ob mice compared with the same fat depot in lean mice. The i.p. administration of leptin in obese ob/ob mice decreases the expression of INHBB. In human adipose tissue, INHBB is reduced by weight loss. In keeping with this, we demonstrate that INHBB expression in murine adipose tissue is decreased in fasting and increased upon refeeding. We show that INHBB is expressed in both the mature adipocyte and the stromal vascular fraction of adipose tissue. INHBB increases with the differentiation of pre-adipocytes into mature adipocytes in the 3T3-L1 cell line. In differentiated 3T3-L1 adipocytes, where receptors to activin have been previously reported, insulin increases the expression of INHBB, while dexamethasone decreases the expression of INHBB when compared with untreated control cells. Taken together, these results suggest that the regulation of INHBB expression in adipose tissue may play a physiological role in energy balance or the insulin insensitivity associated with obesity.