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Journal of Molecular Endocrinology (2009) 42 185-190    DOI: 10.1677/JME-08-0150
© 2009 Society for Endocrinology

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Review

How to study lipidomes

Tuulikki Seppänen-Laakso and Matej Oresic

VTT Technical Research Centre of Finland, PO Box 1000 (Tietotie 2), Espoo FI-02044 VTT, Finland

(Correspondence should be addressed to M Oresic Email: matej.oresic{at}vtt.fi)

Lipidome is loosely defined as the entire spectrum of lipids in a biological system. Given the modern lipidomics platforms for the first time empower us with the ability to obtain a snapshot of complete cellular/organismal lipidome, many surprises and discoveries are likely awaiting us in the area of lipids as related to cellular/organismal physiology. The lipidomics approaches can be applied both as the phenotyping platform and for the hypothesis-driven research aiming to elucidate, e.g., a specific pathway or gene function. Modern lipidomics methods combine the latest mass spectrometry technology and bioinformatics methods with traditional methods such as for sample preparation and lipid extraction.







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