| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Medicine and Bioregulatory Science, Graduate School of Medical Science,2 Department of Molecular Genetics, Medical Institute of Bioregulation and 3 Graduate School of Medical Science, Kyushu University, Maidashi 3-1-1, Higashi-ku, Fukuoka 812-8582, Japan
(Correspondence should be addressed to T Yanase; Email: yanase{at}intmed3.med.kyushu-u.ac.jp)
Steroidogenic factor 1/adrenal 4 binding protein (SF-1/Ad4BP) is an essential nuclear receptor for steroidogenesis as well as for adrenal and gonadal gland development. Mesenchymal bone marrow cells (BMCs) contain pluripotent progenitor cells, which differentiate into multiple lineages. In a previous study, we reported that adenovirus-mediated forced expression of SF-1 could transform mouse primary long-term cultured BMCs into steroidogenic cells. For future clinical application, trials using human BMCs would be indispensable. In this study, we examined whether SF-1 could transform human BMCs into steroidogenic cells and compared the steroid profile of these cellswith that of mouse steroidogenic BMCs. Primary cultured human BMCs infected with adenovirus containing bovine SF-1 cDNA could produce progesterone, corticosterone, cortisol, dehydroepiandrosterone, testosterone, and estradiol. Such a mixed character of adrenal and gonadal steroid production in human BMCs was supported by the expressions of P450scc, 3ß-hydroxysteroid dehydrogenase (3ß-HSD), P450c21, P450c11, P450c17, 17ß-HSD, and P450arom mRNAs. Unlike mouse steroidogenic BMCs, introduction of SF-1 into human BMCs caused dramatic inductions of both ACTH and LH receptors, thus leading to good responsiveness of the cells to ACTH and LH respectively. Importantly, among several factors that are known to be closely associated with adrenal and/or gonadal development, introduction of only SF-1 enabled the human BMCs to express P450scc and to produce cortisol and testosterone, suggesting that SF-1 is truly a master regulator for the production of steroidogenic cells from human BMCs.
This article has been cited by other articles:
|
|
 |
|
  M. Zubair, S. Oka, K. L. Parker, and K.-i. Morohashi Transgenic Expression of Ad4BP/SF-1 in Fetal Adrenal Progenitor Cells Leads to Ectopic Adrenal Formation Mol. Endocrinol., October 1, 2009; 23(10): 1657 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yazawa, Y. Inanoka, T. Mizutani, M. Kuribayashi, A. Umezawa, and K. Miyamoto Liver Receptor Homolog-1 Regulates the Transcription of Steroidogenic Enzymes and Induces the Differentiation of Mesenchymal Stem Cells into Steroidogenic Cells Endocrinology, August 1, 2009; 150(8): 3885 - 3893. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Sakai, H. Terami, S. Suzuki, M. Haga, K. Nomoto, N. Tsuchida, K.-i. Morohashi, N. Saito, M. Asada, M. Hashimoto, et al. Identification of NR5A1 (SF-1/AD4BP) gene expression modulators by large-scale gain and loss of function studies J. Endocrinol., September 1, 2008; 198(3): 489 - 497. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gondo, T. Okabe, T. Tanaka, H. Morinaga, M. Nomura, R. Takayanagi, H. Nawata, and T. Yanase Adipose Tissue-Derived and Bone Marrow-Derived Mesenchymal Cells Develop into Different Lineage of Steroidogenic Cells by Forced Expression of Steroidogenic Factor 1 Endocrinology, September 1, 2008; 149(9): 4717 - 4725. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
