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Endocrinology Centre, William Harvey Research Institute, Bart’s and The London, Queen Mary, University of London, London EC1A 7BE, UK
¹ Institute of Comparative Medicine, University of Glasgow Veterinary School, Glasgow G61 1QH, UK

(Requests for offprints should be addressed to P J King who is now at Molecular Endocrinology Centre, William Harvey Research Institute, John Vane Science Building, Charterhouse Square, London EC1M 6BQ, UK; Email: p.j.king{at}qmul.ac.uk)

The ACTH receptor melanocortin 2 receptor (MC2-R) is a G-protein-coupled receptor principally expressed in the adrenal cortex and the adipocyte, where it stimulates steroidogenesis and lipolysis respectively. The coding region of the murine gene is encoded by a single exon, although three upstream non-coding exons have been documented, one of which is incorporated by alternative splicing in adrenal cells. We have detected a novel transcript in adipocytes, which includes a previously unidentified 86 bp exon upstream of the coding region. This transcript appears with slower kinetics during a time course of differentiation of 3T3-L1 cells and is much more highly expressed in these cells and murine adipose tissues than in the Y1 murine adrenocortical cell line, also it is undetectable in murine foetal testes. Inclusion of this exon extends the 5' UTR to 468 bp and introduces three upstream open reading frames. These are typical features of mRNAs under translational control and imply that the MC2-R gene is regulated both transcriptionally and post-transcriptionally during adipogenesis.

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