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CNRS UMR 6026-Endocrinologie Moléculaire de la Reproduction, Université de Rennes 1, campus de Beaulieu, 35042 Rennes cedex, France
¹ CNRS UMR 6185- Université de Caen, INSERM-Avenir "tPA in the working brain" Centre Cycéron, BP 5229, 14074 Caen cedex, France

(Requests for offprints should be addressed to C Saligaut; Email: Christian.saligaut{at}univ-rennes1.fr)

Numerous studies, both in vivo and in vitro, have reported neuronal differentiating and neuroprotective actions of estrogens. Most of these estrogenic effects are mediated through specific receptors termed estrogen receptors. The aim of this study was to assess the importance of the N-terminal A/B domain of the estrogen receptor-alpha (ER) in its neuronal aspects. Consequently, estrogen effects on (i) the transcriptional activity of target genes, (ii) neuronal differentiation and (iii) neuroprotection in PC12 cells transfected with either a full length form of ER or an A/B domain truncated form (ERCF), have been studied. We demonstrate that the maximal estrogen-induced transcriptional activity of reporter genes requires a full length ER, especially when cells are differentiated. Precisely, the transcriptional activity of ER in differentiated cells relies, predominantly, on the activation function AF-1, located in the A/B domain. Furthermore, in PC12 cells stably expressing ER, 17ß-estradiol markedly enhances the neurite outgrowth triggered by treatment with nerve growth factor and protects cells from oxidative shocks induced by depletion of glutathione. These estrogenic effects are not observed in non-transfected cells and in cells transfected with the truncated ER, devoid of the A/B domain. Altogether, these results underline the importance of the A/B domain of ER in both the differentiating and the neuroprotective effects of estrogens.

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				J. W. Simpkins, E. Perez, Xiaofei Wang, ShaoHua Yang, Yi Wen, and M. Singh The Potential for Estrogens in Preventing Alzheimer's Disease. Therapeutic Advances in Neurological Disorders, January 1, 2009; 2(1): 31 - 49. [Abstract] [PDF]

				Y. Merot, F. Ferriere, L. Gailhouste, G. Huet, F. Percevault, C. Saligaut, and G. Flouriot Different Outcomes of Unliganded and Liganded Estrogen Receptor-{alpha} on Neurite Outgrowth in PC12 Cells Endocrinology, January 1, 2009; 150(1): 200 - 211. [Abstract] [Full Text] [PDF]