HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (8) Citing Articles via Google Scholar Google Scholar Articles by Takizawa, S. Articles by Saida, K. Search for Related Content PubMed PubMed Citation Articles by Takizawa, S. Articles by Saida, K.

¹ Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8566, Japan
² Department of Toxicology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
³ New Energy and Industrial Technology Development Organization (NEDO), 16F, MUZA KAWASAKI Central Tower 1310 Omiya-cho, Saiwai-ku, Kawasaki, Kanagawa 212-8554, Japan
⁴ Human Stress Signal Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Ikeda, Osaka 536-8577, Japan

(Requests for offprints should be addressed to K Saida; email: k.saida{at}aist.go.jp)

Endothelin (ET)-2, an ET family peptide, is highly expressed in intestine. However, the specific distribution and function of ET-2 remain unknown. We elucidated the expression profile and localization of ET-2 in mouse gastrointestinal tract. Real-time PCR analysis revealed that ET-2 gene expression in the gastrointestinal tract of healthy animals was relatively high in the colon. Immunohistochemical analysis revealed ET-2-like immunoreactivity mainly in epithelial cells of the mucosa throughout the intestinal tract of healthy animals. Intracellularly, ET-2 was concentrated close to the basement membrane of intestinal epithelial cells. A weak ET-2-like immunoreactivity was also localized to some neurofibers and the myenteric plexus of the muscle layer, coexpressing with vasoactive intestinal peptide. ET-2-like immunoreactivity was also detected at Brunner’s glands of the duodenum and follicle-associated epithelium of Peyer’s patch. In contrast, ET-1-like immunoreactivity was uniformly distributed in epithelial cells. In dextran sulfate sodium (DSS)-induced colitis, colonic ET-2 was upregulated during the late stage of DSS treatment. These results suggest that in intestinal epithelial cells ET-2 could be secreted into the lamina propria and the dome region in Peyer’s patch, and that it might modulate immune cells in these sites for mucosal defense.

This article has been cited by other articles:

				J. Kalabis, G. Li, M. Fukunaga-Kalabis, A. K. Rustgi, and M. Herlyn Endothelin-3 stimulates survival of goblet cells in organotypic cultures of fetal human colonic epithelium Am J Physiol Gastrointest Liver Physiol, December 1, 2008; 295(6): G1182 - G1189. [Abstract] [Full Text] [PDF]

				T. Rankinen, T. Church, T. Rice, N. Markward, A. S. Leon, D. C. Rao, J. S. Skinner, S. N. Blair, and C. Bouchard Effect of Endothelin 1 Genotype on Blood Pressure Is Dependent on Physical Activity or Fitness Levels Hypertension, December 1, 2007; 50(6): 1120 - 1125. [Abstract] [Full Text] [PDF]

				H. Wang, J. Quan, E.-I. Kotake-Nara, T. Uchide, T. Andoh, and K. Saida cDNA Cloning and Sequence Analysis of Preproendothelin-1 (PPET-1) from Salmon, Oncorhynchus keta. Experimental Biology and Medicine, June 1, 2006; 231(6): 709 - 712. [Abstract] [Full Text] [PDF]