| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Human Anatomy and Cell Science, University of Manitoba, Winnipeg, Manitoba, Canada
2 Department of Immunology, Martin Luther University, Halle-Wittenberg, Germany
3 Department of Obstetrics and Gynecology, University of Aberdeen, Aberdeen, UK
4 Department of Anatomy, RWTH Aachen, Germany
5 Department of Dermatology, RWTH Aachen, Germany
6 Department of Analytical and Environmental Chemistry, Martin Luther University, Halle-Wittenberg, Germany
7 Department of Pathology, Martin Luther University, Halle-Wittenberg, Germany
(Requests for offprints should be addressed to T Klonisch, Department of Human Anatomy and Cell Science, Faculty of Medicine, 130 Basic Medical Sciences, 730 Williams Avenue, University of Manitoba, Winnipeg, Manitoba, R3E 0W3, Canada; Email: klonisch{at}cc.umanitoba.ca)
Information on the regulation of steroid hormone receptors and their distinct functions within the human endometrial epithelium is largely unavailable. We have immortalized human primary endometrial epithelial cells (EECs) isolated from a normal proliferative phase endometrium by stably transfecting the catalytic subunit (hTERT) of the human telomerase complex and cultured these hTERT-EECs now for over 350 population doublings. Active hTERT was detected in hTERT-EECs employing the telomerase repeat amplification assay protocol. hTERT-EECs revealed a polarized, non-invasive epithelial phenotype with apical microvilli and production of a basal lamina when grown on a three-dimensional collagen–fibroblast lattice. Employing atomic force microscopy, living hTERT-EECs were shown to produce extracellular matrix (ECM) components and ECM secretion was modified by estrogen and progesterone (P4). hTERT-EECs expressed inducible and functional endogenous estrogen receptor-alpha (ER-alpha) as demonstrated by estrogen response element reporter assays and induction of P4 receptor (PR). P4 treatment down-regulated PR expression, induced MUC-1 gene activity and resulted in increased ER-beta transcriptional activity. Gene activities of cytokines and their receptors interleukin (IL)-6, leukemia inhibitory factor (LIF), IL-11 and IL-6 receptor (IL6-R), LIF receptor and gp130 relevant to implantation revealed a 17 beta-estradiol (E2)-mediated up-regulation of IL-6 and an E2- and P4-mediated up-regulation of IL6-R in hTERT-EECs. Thus, hTERT-EECs may be regarded as a novel in vitro model to investigate the role of human EECs in steroid hormone-dependent normal physiology and pathologies, including implantation failure, endometriosis and endometrial cancer.
This article has been cited by other articles:
|
|
 |
|
  A.E. King, F. Collins, T. Klonisch, J.-M. Sallenave, H.O.D. Critchley, and P.T.K. Saunders An additive interaction between the NF{kappa}B and estrogen receptor signalling pathways in human endometrial epithelial cells Hum. Reprod., February 1, 2010; 25(2): 510 - 518. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shimizu, T. Takeuchi, S. Mita, K. Mizuguchi, T. Kiyono, M. Inoue, and S. Kyo Dienogest, a synthetic progestin, inhibits the proliferation of immortalized human endometrial epithelial cells with suppression of cyclin D1 gene expression Mol. Hum. Reprod., October 1, 2009; 15(10): 693 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. DIVELLA, S. TOMMASI, R. LACALAMITA, A. DANIELE, I. ABBATE, V. M. GARRISI, E. SAVINO, M. COVIELLO, V. RUBINI, G. SIMONE, et al. Circulating hTERT DNA in Early Breast Cancer Anticancer Res, July 1, 2009; 29(7): 2845 - 2849. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. King, A. W. Horne, S. Hombach-Klonisch, J. I. Mason, and H. O.D. Critchley Differential expression and regulation of nuclear oligomerization domain proteins NOD1 and NOD2 in human endometrium: a potential role in innate immune protection and menstruation Mol. Hum. Reprod., May 1, 2009; 15(5): 311 - 319. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.W. Horne, W.C. Duncan, A.E. King, S. Burgess, P.C. Lourenco, P. Cornes, P. Ghazal, A.R. Williams, L. Udby, and H.O.D. Critchley Endometrial cysteine-rich secretory protein 3 is inhibited by human chorionic gonadotrophin, and is increased in the decidua of tubal ectopic pregnancy Mol. Hum. Reprod., May 1, 2009; 15(5): 287 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Wanggren, P.G. Lalitkumar, F. Hambiliki, B. Stabi, K. Gemzell-Danielsson, and A. Stavreus-Evers Leukaemia inhibitory factor receptor and gp130 in the human Fallopian tube and endometrium before and after mifepristone treatment and in the human preimplantation embryo Mol. Hum. Reprod., June 1, 2007; 13(6): 391 - 397. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Achache and A. Revel Endometrial receptivity markers, the journey to successful embryo implantation Hum. Reprod. Update, November 1, 2006; 12(6): 731 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hombach-Klonisch, P. Pocar, J. Kauffold, and T. Klonisch Dioxin Exerts Anti-estrogenic Actions in a Novel Dioxin-Responsive Telomerase-Immortalized Epithelial Cell Line of the Porcine Oviduct (TERT-OPEC) Toxicol. Sci., April 1, 2006; 90(2): 519 - 528. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
