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Department of Zoology, Oregon State University, Corvallis, OR 97331, USA

(Requests for offprints should be addressed to F L Moore; Email: mooref{at}science.oregonstate.edu)

We have cloned and characterized an opioid receptor-like (ORL1; also referred to as NOP) receptor from a urodele amphibian, the rough-skinned newt Taricha granulosa The cDNA clone encodes a protein of 368 amino acids that contains the seven hydrophobic domains characteristic of G-protein-coupled receptors, and has the highest sequence identity to the frog (Rana pipiens) nociceptin-like and human ORL1 opioid receptors (79.6 and 68.4%, respectively). Saturation binding assays on membranes from COS-7 cells transiently expressing the newt ORL1 (nORL) receptor revealed a single, high-affinity (estimated K_d, 0.1974 nM) binding site for the ORL1-specific agonist [³H]orphanin FQ analog ([³H]oFQ). In competition binding assays, the [³H]oFQ-binding site, like the mammalian ORL1 receptor, had no affinity for the non-selective opioid receptor antagonist naloxone, the -selective agonists U69593 and U50488, or the µ- and -selective opioid receptor agonists DAMGO and DPDPE, respectively. However, the nORL receptor displayed higher affinities for the -selective agonists dynorphin A (1–13), dynorphin B, and dynorphin A (1–8) (K_i values, 2.8, 151.8, and 183.0 nM, respectively) than its mammalian homologue. The tissue distribution of the nORL receptor, as determined by reverse transcriptase PCR, was also found to differ from reports on the mammalian ORL1 receptor, with mRNA detected in brain, spinal cord, and lung, but not detected in a number of other peripheral tissues reported to express the receptor in mammals. This is the first report describing the expression and characterization of an amphibian ORL1 receptor, and contributes to our understanding of the evolution of the opioid system.

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				C S. Bradford, E. A Walthers, B. T Searcy, and F. L Moore Cloning, heterologous expression and pharmacological characterization of a kappa opioid receptor from the brain of the rough-skinned newt, Taricha granulosa J. Mol. Endocrinol., June 1, 2005; 34(3): 809 - 823. [Abstract] [Full Text] [PDF]