| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
For many, analysis of a microarray experiment starts with a spreadsheet of expression levels. While great attention is duly paid to RNA extraction, preparation and hybridization, relatively little care is devoted to extraction of expression levels from the fluorescent image. By delegating this step to a click of the mouse the exact extraction process is masked and researchers may be unwittingly compromising their data. In this review, we describe the most common mistakes committed on the path from the image to the spreadsheet and their impact on data quality. Remedies are further proposed for most of the popular microarray platforms in use today.
This article has been cited by other articles:
|
|
 |
|
  S. A. Boehme, E. P. Chen, K. Franz-Bacon, R. Sasik, L. J. Sprague, T. W. Ly, G. Hardiman, and K. B. Bacon Antagonism of CRTH2 ameliorates chronic epicutaneous sensitization-induced inflammation by multiple mechanisms Int. Immunol., January 1, 2009; 21(1): 1 - 17. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Boehme, K. Franz-Bacon, E. P. Chen, R. Sasik, L. J. Sprague, T. W. Ly, G. Hardiman, and K. B. Bacon A small molecule CRTH2 antagonist inhibits FITC-induced allergic cutaneous inflammation Int. Immunol., January 1, 2009; 21(1): 81 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. W. Lukacs, A. A. Berlin, K. Franz-Bacon, R. Sasik, L. J. Sprague, T. W. Ly, G. Hardiman, S. A. Boehme, and K. B. Bacon CRTH2 antagonism significantly ameliorates airway hyperreactivity and downregulates inflammation-induced genes in a mouse model of airway inflammation Am J Physiol Lung Cell Mol Physiol, November 1, 2008; 295(5): L767 - L779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Pellicciotta, X. Cortez-Gonzalez, R. Sasik, Y. Reiter, G. Hardiman, P. Langlade-Demoyen, and M. Zanetti Presentation of Telomerase Reverse Transcriptase, a Self-Tumor Antigen, is Down-regulated by Histone Deacetylase Inhibition Cancer Res., October 1, 2008; 68(19): 8085 - 8093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Nguyen, J. A. Bonzo, S. Chen, S. Chouinard, M. J. Kelner, G. Hardiman, A. Belanger, and R. H. Tukey Disruption of the Ugt1 Locus in Mice Resembles Human Crigler-Najjar Type I Disease J. Biol. Chem., March 21, 2008; 283(12): 7901 - 7911. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Simmen, R. Xiao, M. C. Velarde, R. D. Nicholson, M. T. Bowman, Y. Fujii-Kuriyama, S. P. Oh, and R. C. M. Simmen Dysregulation of intestinal crypt cell proliferation and villus cell migration in mice lacking Kruppel-like factor 9 Am J Physiol Gastrointest Liver Physiol, June 1, 2007; 292(6): G1757 - G1769. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Schwartz, I. Engel, M. Fallahi-Sichani, H. T. Petrie, and C. Murre Gene expression patterns define novel roles for E47 in cell cycle progression, cytokine-mediated signaling, and T lineage development PNAS, June 27, 2006; 103(26): 9976 - 9981. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. J. Glatt, I. P. Everall, W. S. Kremen, J. Corbeil, R. Sasik, N. Khanlou, M. Han, C.-C. Liew, and M. T. Tsuang Comparative gene expression analysis of blood and brain provides concurrent validation of SELENBP1 up-regulation in schizophrenia PNAS, October 25, 2005; 102(43): 15533 - 15538. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. A White and L. A Salamonsen A guide to issues in microarray analysis: application to endometrial biology Reproduction, July 1, 2005; 130(1): 1 - 13. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
