Glucose-6-phosphatase (G6Pase) catalyzes the final step in the gluconeogenic and glycogenolytic pathways, the hydrolysis of glucose-6-phosphate (G6P) to glucose and phosphate. This paper describes the identification and characterization of a cDNA and the gene encoding the mouse ubiquitously expressed G6Pase catalytic subunit-related protein (UGRP). The open reading frame of this UGRP cDNA encodes a protein (346 amino acids (aa); Mr 38,755) that shares 36% overall identity (56% similarity) with the mouse G6Pase catalytic subunit (357 aa; Mr 40,454). UGRP exhibits a similar predicted transmembrane topology and conservation of many of the catalytically important residues with the G6Pase catalytic subunit; however, unlike the G6Pase catalytic subunit, UGRP does not catalyze G6P hydrolysis and does not contain a carboxy-terminal di-lysine endoplasmic reticulum retention signal. UGRP mRNA was detected by RNA blot analysis in every mouse tissue examined with the highest expression in heart, brain, testis and kidney. Database analysis showed that the mouse UGRP gene is composed of six exons, spans approximately 4.2 kbp of genomic DNA and is located on chromosome 11 along with the G6Pase catalytic subunit gene. The UGRP gene transcription start sites were mapped by primer extension analysis, and the activity of the mouse UGRP gene promoter was analyzed using luciferase fusion gene constructs. In contrast to the G6Pase catalytic subunit gene promoter, the UGRP promoter was highly active in all cell lines examined.

This article has been cited by other articles:

				J. C. Hutton and R. M. O'Brien Glucose-6-phosphatase Catalytic Subunit Gene Family J. Biol. Chem., October 23, 2009; 284(43): 29241 - 29245. [Abstract] [Full Text] [PDF]

				C. C Martin, B. P Flemming, Y. Wang, J. K Oeser, and R. M O'Brien Foxa2 and MafA regulate islet-specific glucose-6-phosphatase catalytic subunit-related protein gene expression J. Mol. Endocrinol., November 1, 2008; 41(5): 315 - 328. [Abstract] [Full Text] [PDF]

				Y. Wang, B. P. Flemming, C. C. Martin, S. R. Allen, J. Walters, J. K. Oeser, J. C. Hutton, and R. M. O'Brien Long-Range Enhancers Are Required to Maintain Expression of the Autoantigen Islet-Specific Glucose-6-Phosphatase Catalytic Subunit Related Protein in Adult Mouse Islets In Vivo Diabetes, January 1, 2008; 57(1): 133 - 141. [Abstract] [Full Text] [PDF]

				D. Azzout-Marniche, C. Gaudichon, C. Blouet, C. Bos, V. Mathe, J.-F. Huneau, and D. Tome Liver glyconeogenesis: a pathway to cope with postprandial amino acid excess in high-protein fed rats? Am J Physiol Regulatory Integrative Comp Physiol, April 1, 2007; 292(4): R1400 - R1407. [Abstract] [Full Text] [PDF]

				Y. Wang, J. K. Oeser, C. Yang, S. Sarkar, S. I. Hackl, A. H. Hasty, O. P. McGuinness, W. Paradee, J. C. Hutton, D. R. Powell, et al. Deletion of the Gene Encoding the Ubiquitously Expressed Glucose-6-phosphatase Catalytic Subunit-related Protein (UGRP)/Glucose-6-phosphatase Catalytic Subunit-beta Results in Lowered Plasma Cholesterol and Elevated Glucagon J. Biol. Chem., December 29, 2006; 281(52): 39982 - 39989. [Abstract] [Full Text] [PDF]

				A. Ghosh, Y. Y. Cheung, B. C. Mansfield, and J. Y. Chou Brain Contains a Functional Glucose-6-Phosphatase Complex Capable of Endogenous Glucose Production J. Biol. Chem., March 25, 2005; 280(12): 11114 - 11119. [Abstract] [Full Text] [PDF]