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Journal of Molecular Endocrinology (1989) 3, 163-166    DOI: 10.1677/jme.0.0030163
© 1989 Society for Endocrinology

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Cytoplasmic pH in cultured porcine thyroid cells: alkalinization by Ca2+

N. Takasu, I. Komiya, Y. Nagasawa, T. Asawa, Y. Shimizu and T. Yamada

The role of calcium in cytoplasmic pH (pHi) changes was studied using 2',7'-bis(2-carboxyethyl)-5-(and 6-)carboxyfluorescein, an internalized fluorescent pH indicator, in cultured porcine thyroid cells. The Ca2+ ionophores A23187 [GenBank] and ionomycin stimulated thyroid cell alkalinization. An increase in cytoplasmic free calcium resulted in activation of Na+/H+ exchange which alkalinized the cells.







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