JME
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

DOI: 10.1677/jme.0.0290073
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (17)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mao, J
Right arrow Articles by Seyfert, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mao, J
Right arrow Articles by Seyfert, H.
Journal of Molecular Endocrinology, Vol 29, Issue 1, 73-88
Copyright © 2002 by Society for Endocrinology

Articles

STAT5 binding contributes to lactational stimulation of promoter III expressing the bovine acetyl-CoA carboxylase alpha-encoding gene in the mammary gland

J Mao, AJ Molenaar, TT Wheeler, and HM Seyfert


Activity of acetyl-CoA carboxylase (ACC)-alpha is rate limiting for de novo synthesis of fatty acids. The encoding gene is expressed by three different promoters. We characterized promoter III (PIII) from cow, previously only known from sheep. Quantitation of transcripts by RNAse protection assays and real time PCR revealed that PIII is primarily expressed and strongly induced ( approximately 28-fold) in the lactating mammary gland. PIII transcripts are expressed in mammary epithelial cells (MEC) as shown by in situ hybridization. A 2999 bp segment of the PIII promoter conferred prolactin and dexamethasone inducibility to a luciferase reporter gene in stably transfected mouse MEC cells. Lactogenic induction was abolished if a unique signal transducer and activator of transcription (STAT)-binding site at position -797 was inactivated by two point mutations. An oligonucleotide probe harboring this STAT-site specifically bound nuclear proteins from the lactating mammary gland. Binding was abolished by those two point mutations and super-shift analyses showed that STAT5A factors are present in this complex. Hence, prolactin, acting through STAT5, contributes to the activation of ACC expression in the milk producing cells of the lactating mammary gland. We discuss that STAT5 might be important in determining the milk composition by coordinating fatty acid and protein synthesis during lactation.


This article has been cited by other articles:


Home page
 J Mol EndocrinolHome page
J. Vanselow, W. Yang, J. Herrmann, H. Zerbe, H.-J. Schuberth, W. Petzl, W. Tomek, and H.-M. Seyfert
DNA-remethylation around a STAT5-binding enhancer in the {alpha}S1-casein promoter is associated with abrupt shutdown of {alpha}S1-casein synthesis during acute mastitis
J. Mol. Endocrinol., December 1, 2006; 37(3): 463 - 477.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
O. Cobanoglu, I. Zaitoun, Y. M. Chang, G. E. Shook, and H. Khatib
Effects of the signal transducer and activator of transcription 1 (STAT1) gene on milk production traits in Holstein dairy cattle.
J Dairy Sci, November 1, 2006; 89(11): 4433 - 4437.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
W. Liu, S. C. Degner, and D. F. Romagnolo
Trans-10, Cis-12 Conjugated Linoleic Acid Inhibits Prolactin-Induced Cytosolic NADP+-Dependent Isocitrate Dehydrogenase Expression in Bovine Mammary Epithelial Cells
J. Nutr., November 1, 2006; 136(11): 2743 - 2747.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
C. M. Murrieta, B. W. Hess, E. J. Scholljegerdes, T. E. Engle, K. L. Hossner, G. E. Moss, and D. C. Rule
Evaluation of milk somatic cells as a source of mRNA for study of lipogenesis in the mammary gland of lactating beef cows supplemented with dietary high-linoleate safflower seeds
J Anim Sci, September 1, 2006; 84(9): 2399 - 2405.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. GenomicsHome page
R. Furbass, A. Winter, R. Fries, and C. Kuhn
Alleles of the bovine DGAT1 variable number of tandem repeat associated with a milk fat QTL at chromosome 14 can stimulate gene expression.
Physiol Genomics, March 13, 2006; 25(1): 116 - 120.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
O. M. Sinilnikova, S. M. Ginolhac, C. Magnard, M. Leone, O. Anczukow, D. Hughes, K. Moreau, D. Thompson, C. Coutanson, J. Hall, et al.
Acetyl-CoA carboxylase {alpha} gene and breast cancer susceptibility
Carcinogenesis, December 1, 2004; 25(12): 2417 - 2424.
[Abstract] [Full Text] [PDF]

Home page
 CVIHome page
T. Goldammer, H. Zerbe, A. Molenaar, H.-J. Schuberth, R. M. Brunner, S. R. Kata, and H.-M. Seyfert
Mastitis Increases Mammary mRNA Abundance of {beta}-Defensin 5, Toll-Like-Receptor 2 (TLR2), and TLR4 but Not TLR9 in Cattle
Clin. Vaccine Immunol., January 1, 2004; 11(1): 174 - 185.
[Abstract] [Full Text] [PDF]

Home page
 J. Histochem. Cytochem.Home page
A. Molenaar, J. Mao, K. Oden, and H.-M. Seyfert
All Three Promoters of the Acetyl-Coenzyme A-Carboxylase {alpha}-encoding Gene Are Expressed in Mammary Epithelial Cells of Ruminants
J. Histochem. Cytochem., August 1, 2003; 51(8): 1073 - 1081.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Mao, S. S. Chirala, and S. J. Wakil
Human acetyl-CoA carboxylase 1 gene: Presence of three promoters and heterogeneity at the 5'-untranslated mRNA region
PNAS, June 24, 2003; 100(13): 7515 - 7520.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 2002 by the Society for Endocrinology.