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DOI: 10.1677/jme.0.0200375
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Journal of Molecular Endocrinology, Vol 20, Issue 3, 375-380
Copyright © 1998 by Society for Endocrinology

Articles

A novel oestrogen-regulated gene in human breast cancer cells identified by differential display

R Stephen, D Corcoran, and PD Darbre


Screening by differential display of oestrogen-sensitive MCF7 human breast cancer cells grown in the short-term (6 days) and long-term (70 weeks) absence of oestrogen has led to the identification of a new oestrogen-regulated mRNA. The cDNA isolated by differential display has 100% homology from nucleotides 615 to 859 of the published sequence for the mRNA for human megakaryocyte CD63 antigen but has a 3' tail extended by 23 nucleotides. Northern blotting has confirmed that this mRNA is regulated by oestrogen in both MCF7 and T47D human breast cancer cell lines.


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 Mol. Endocrinol.Home page
J. S. Carroll and M. Brown
Estrogen Receptor Target Gene: An Evolving Concept
Mol. Endocrinol., August 1, 2006; 20(8): 1707 - 1714.
[Abstract] [Full Text] [PDF]


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