| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
Repression of basal transcription of a 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) responsive 25-hydroxyvitamin D3-24-hydroxylase (CYP24) promoter construct as observed in kidney cells in the absence of ligand and this repression was dependent on a functional vitamin D response element (VDRE). Basal repression was also seen with a construct where a consensus DR-3-type VDRE was fused to the thymidine kinase promoter. Expression of a dominant negative vitamin D receptor (VDR) isoform that strongly bound to the VDRE motif in the CYP24 promoter ablated basal repression. This VDR isoform lacked sequence in the hinge- and ligand-binding domains implicating one or both of these domains in basal repression. It is well known that thyroid hormone and retinoic acid receptors silence basal transcription of target genes in the absence of ligands and this repressor function can be mediated by the nuclear receptor corepressor N-CoR. Two variants of N-CoR have been described, RIP13a and RIP13delta1. N-CoR and the variants contain two receptor interaction domains, ID-I and ID-II, which are identical except region ID-II in RIP13delta1 has an internal deletion. We have used the mammalian two hybrid system to investigate whether VDR, in the absence of ligand 1,25-(OH)2D3, can interact with these domains. The data showed that unliganded VDR does not interact with either ID-I or ID-II from RIP13a and RIP13delta1, but does interact strongly with a composite domain of ID-I and ID-II from RIP13delta1 (but not from RIP13a) and this strong interaction is abrogated in the presence of ligand. This finding implicates RIP13delta1 in VDR-dependent basal repression of the promoter constructs under investigation. However, over-expression of RIP13delta1 in kidney cell lines did not alter basal expression of the CYP24 promoter construct. It is concluded that either the level of endogenous RIP13delta1 in these kidney cells permits maximal repression or that repression occurs by a mechanism that is independent of RIP13delta1. Alternatively, repression may be dependent on RIP13delta1 but requires an additional cofactor that is limiting in these cells.
This article has been cited by other articles:
|
|
 |
|
  R. Sanchez-Martinez, A. Zambrano, A. I. Castillo, and A. Aranda Vitamin D-Dependent Recruitment of Corepressors to Vitamin D/Retinoid X Receptor Heterodimers Mol. Cell. Biol., June 1, 2008; 28(11): 3817 - 3829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. A. Jonas, N. Varlakhanova, F. Hayakawa, M. Goodson, and M. L. Privalsky Response of SMRT (Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor) and N-CoR (Nuclear Receptor Corepressor) Corepressors to Mitogen-Activated Protein Kinase Kinase Kinase Cascades Is Determined by Alternative mRNA Splicing Mol. Endocrinol., August 1, 2007; 21(8): 1924 - 1939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Frasor, J. M. Danes, C. C. Funk, and B. S. Katzenellenbogen Estrogen down-regulation of the corepressor N-CoR: Mechanism and implications for estrogen derepression of N-CoR-regulated genes PNAS, September 13, 2005; 102(37): 13153 - 13157. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Moehren, U. Dressel, C. A. Reeb, S. Vaisanen, T. W. Dunlop, C. Carlberg, and A. Baniahmad The highly conserved region of the co-repressor Sin3A functionally interacts with the co-repressor Alien Nucleic Acids Res., June 1, 2004; 32(10): 2995 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Ueki and M. J. Hayman Signal-dependent N-CoR Requirement for Repression by the Ski Oncoprotein J. Biol. Chem., June 27, 2003; 278(27): 24858 - 24864. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Barry, G. M. Leong, W. B. Church, L. L. Issa, J. A. Eisman, and E. M. Gardiner Interactions of SKIP/NCoA-62, TFIIB, and Retinoid X Receptor with Vitamin D Receptor Helix H10 Residues J. Biol. Chem., February 28, 2003; 278(10): 8224 - 8228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. P. Dwivedi, C. S. T. Hii, A. Ferrante, J. Tan, C. J. Der, J. L. Omdahl, H. A. Morris, and B. K. May Role of MAP Kinases in the 1,25-Dihydroxyvitamin D3-induced Transactivation of the Rat Cytochrome P450C24 (CYP24) Promoter. SPECIFIC FUNCTIONS FOR ERK1/ERK2 AND ERK5 J. Biol. Chem., August 9, 2002; 277(33): 29643 - 29653. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. POLLY, M. HERDICK, U. MOEHREN, A. BANIAHMAD, T. HEINZEL, and C. CARLBERG VDR-Alien: a novel, DNA-selective vitamin D3 receptor-corepressor partnership FASEB J, July 1, 2000; 14(10): 1455 - 1463. [Abstract] [Full Text]
|
|
|
|
|
|
P. P. Dwivedi, J. L. Omdahl, I. Kola, D. A. Hume, and B. K. May Regulation of Rat Cytochrome P450C24 (CYP24) Gene Expression. EVIDENCE FOR FUNCTIONAL COOPERATION OF Ras-ACTIVATED Ets TRANSCRIPTION FACTORS WITH THE VITAMIN D RECEPTOR IN 1,25-DIHYDROXYVITAMIN D3-MEDIATED INDUCTION J. Biol. Chem., January 7, 2000; 275(1): 47 - 55. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Racz and J. Barsony Hormone-dependent Translocation of Vitamin D Receptors Is Linked to Transactivation J. Biol. Chem., July 2, 1999; 274(27): 19352 - 19360. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Bailey, M. Downes, P. Lau, J. Harris, S. L. Chen, Y. Hamamori, V. Sartorelli, and G. E. O. Muscat The Nuclear Receptor Corepressor N-CoR Regulates Differentiation: N-CoR Directly Interacts with MyoD Mol. Endocrinol., July 1, 1999; 13(7): 1155 - 1168. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
