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DOI: 10.1677/jme.0.0200151
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Journal of Molecular Endocrinology, Vol 20, Issue 1, 151-156
Copyright © 1998 by Society for Endocrinology

Articles

Suppression subtractive hybridization (SSH) identifies prolactin stimulation of p38 MAP kinase gene expression in Nb2 T lymphoma cells: molecular cloning of rat p38 MAP kinase

E Nemeth, C Bole-Feysot, and LS Tashima


Suppression Subtractive Hybridization (SSH) has been used to compare rat Nb2 cells treated with prolactin for 1 hour with untreated cells. This new method for identifying differentially expressed genes showed that the mRNAs for at least three genes were elevated by such treatment, including a p38 mitogen activated protein (MAP) kinase. The p38 MAP kinase was cloned and the full length cDNA sequence was determined.


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