| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
A polymorphism of the gene encoding the human angiotensin I-converting enzyme (ACE), which is defined by an insertion/deletion polymorphism in intron 16, has been identified as a candidate genetic locus in the development of cardiovascular and renal disease.
We have demonstrated that the accuracy of ACE genotyping is critically dependent on the strategy of the PCR used in typing. Of 1238 individuals genotyped by a standard method, 335 were typed as DD, 645 as DI and 258 as II. However, when DD individuals were retyped using modified methods (including either 5% dimethyl sulphoxide, or a 'hot start') 35 of the original 335 samples (10·5%) were retyped as DI.
In approximately half of these mistyped samples, PCR amplification was assessed as inefficient by the absence of a third faint heteroduplex band in a control ID sample: when the assay was repeated without any modifications, the mistyped samples were correctly genotyped. In the remainder, mistyping persisted. In these cases, the use of a third 'nested' PCR primer specific for the I allele was required for successful genotyping, providing a more reliable strategy without the need for further modification to the PCR technique. Our results suggest that the triple primer approach is the method of choice for accurate ACE genotyping.
This article has been cited by other articles:
|
|
 |
|
  P. M. Jimenez, C. Conde, A. Casanegra, C. Romero, A. Hugo Tabares, and M. Orias Association of ACE genotype and predominantly diastolic hypertension: a preliminary study Journal of Renin-Angiotensin-Aldosterone System, March 1, 2007; 8(1): 42 - 44. [Abstract] [PDF]
|
|
|
|
 |
|
 H Kanazawa, K Hirata, and J Yoshikawa Effects of captopril administration on pulmonary haemodynamics and tissue oxygenation during exercise in ACE gene subtypes in patients with COPD: a preliminary study Thorax, July 1, 2003; 58(7): 629 - 631. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Kanazawa, T. Otsuka, K. Hirata, and J. Yoshikawa Association Between the Angiotensin-Converting Enzyme Gene Polymorphisms and Tissue Oxygenation During Exercise in Patients With COPD Chest, March 1, 2002; 121(3): 697 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Jeron, C Hengstenberg, S Engel, H Lowel, G.A.J Riegger, H Schunkert, and S Holmer The D-allele of the ACE polymorphism is related to increased QT dispersion in 609 patients after myocardial infarction Eur. Heart J., April 2, 2001; 22(8): 663 - 668. [Abstract] [PDF]
|
|
|
|
 |
|
 C. J. Clark, E. Davies, N. H. Anderson, R. Farmer, E. C. Friel, R. Fraser, and J. M. C. Connell {{alpha}}-Adducin and Angiotensin I-Converting Enzyme Polymorphisms in Essential Hypertension Hypertension, December 1, 2000; 36(6): 990 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. KANAZAWA, T. OKAMOTO, K. HIRATA, and J. YOSHIKAWA Deletion Polymorphisms in the Angiotensin Converting Enzyme Gene Are Associated with Pulmonary Hypertension Evoked by Exercise Challenge in Patients with Chronic Obstructive Pulmonary Disease Am. J. Respir. Crit. Care Med., October 1, 2000; 162(4): 1235 - 1238. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. R. M. Osterop, M. J. M. Kofflard, L. A. Sandkuijl, F. J. t. Cate, R. Krams, M. A. D. H. Schalekamp, and A. H. J. Danser AT1 Receptor A/C1166 Polymorphism Contributes to Cardiac Hypertrophy in Subjects With Hypertrophic Cardiomyopathy Hypertension, November 1, 1998; 32(5): 825 - 830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hibi, T. Ishigami, K. Kimura, M. Nakao, T. Iwamoto, K. Tamura, T. Nemoto, T. Shimizu, Y. Mochida, H. Ochiai, et al. Angiotensin-Converting Enzyme Gene Polymorphism Adds Risk for the Severity of Coronary Atherosclerosis in Smokers Hypertension, September 1, 1997; 30(3): 574 - 579. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
