HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Vertongen, p Articles by Robberecht, P Search for Related Content PubMed PubMed Citation Articles by Vertongen, p Articles by Robberecht, P

The expression of the pituitary adenylate cyclase-activating polypeptide/vasoactive intestinal polypeptide (PACAP/VIP) receptor subtypes was evaluated in the normal rat pituitary gland and in different rat spontaneous transplantable SMtTW tumours (SMtTW₂ which expresses prolactin (PRL), SMtTW₁₀ which expresses GH and SMtTW₃ which expresses both PRL and GH) by measurement of PACAP/VIP-stimulated adenylate cyclase activity and detection of the presence of mRNA coding for the different receptor forms. In normal glands, the order of potency of the peptides suggested that adenylate cyclase activity was mediated through interaction with PACAP selective receptors (PACAP I receptors); mRNAs coding for the PACAP I receptor, but also for the PACAP II VIP₂ receptor, were detected. In SMtTW₂ tumours, the functional response was close to that observed in the presence of PACAP II VIP₂ receptors; mRNAs coding for PACAP I and PACAP II VIP₁ and PACAP II VIP₂ receptors were detected. In the SMtTW₁₀ tumours, the functional response was complex but compatible with the involvement of PACAP I and PACAP II receptors; mRNAs coding for the PACAP I and PACAP II VIP₁ receptors were detected. In the SMtTW₃ tumour, the profile was similar to that of the normal pituitary gland and the mRNA coding for the PACAP I receptor only was detected. Thus, while the control of normal pituitary gland adenylate cyclase activity by PACAP and VIP was mediated by PACAP-selective receptors, in spontaneous transplantable tumours a variable profile was observed and PACAP, as well as VIP₁ and VIP₂ receptors, may contribute to the responses.

This article has been cited by other articles:

				M. G. Juarranz, J. Van Rampelbergh, P. Gourlet, P. De Neef, J. Cnudde, P. Robberecht, and M. Waelbroeck Different Vasoactive Intestinal Polypeptide Receptor Domains Are Involved in the Selective Recognition of Two VPAC2-Selective Ligands Mol. Pharmacol., December 1, 1999; 56(6): 1280 - 1287. [Abstract] [Full Text]

				J. Trouillas, P. Chevallier, C. Remy, F. Rajas, R. Cohen, A. Calle, E. L. Hooghe-Peters, and B. Rousset Differential Actions of the Dopamine Agonist Bromocriptine on Growth of SMtTW Tumors Exhibiting a Prolactin and/or a Somatotroph Cell Phenotype: Relation to Dopamine D2 Receptor Expression Endocrinology, January 1, 1999; 140(1): 13 - 21. [Abstract] [Full Text]