HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spencer, T E Articles by Bazer, F W Search for Related Content PubMed PubMed Citation Articles by Spencer, T E Articles by Bazer, F W

This study determined the effects of intrauterine injections of recombinant ovine interferon- (roIFN-; 2 x 10⁷ antiviral units/day) or control proteins (6 mg/day) from day 11 to day 14 post-oestrus (oestrus=day 0) on endometrial expression of receptors for oestrogen, progesterone and oxytocin in cyclic ewes. Plasma concentrations of progesterone were greater on day 15 in ewes receiving roIFN- compared with control proteins (P<0·02, treatment x day). Ewes injected with roIFN- had lower endometrial levels of oestrogen receptor mRNA (P<0·10) and protein (P<0·01) on day 15 compared with ewes receiving control proteins. In situ hybridization analysis indicated that oestrogen receptor mRNA was more abundant in the luminal and glandular epithelium of control ewes compared with roIFN--treated ewes. Immunoreactive oestrogen receptor was also present in the luminal and glandular epithelium of control, but not roIFN--treated ewes. Endometrial levels of progesterone receptor mRNA and protein were not different (P>0·10) between control and roIFN--treated ewes. In situ hybridization analyses indicated that progesterone receptor mRNA abundance was low in endometrial epithelium and stroma of both control and roIFN--injected ewes. Immunoreactive progesterone receptors were present in the endometrial stroma and epithelium of control ewes, but confined to the stroma of roIFN--treated ewes. Oxytocin receptor density was lower (P<0·10) in the endometrium of ewes injected with roIFN- than control proteins; however, oxytocin receptor affinity was not affected (P>0·10) by treatment. Concentrations of 13,14-dihydro-15-keto-prostaglandin F₂ (PGFM) were not increased by exogenous oxytocin administration in control and roIFN--treated ewes on days 10 or 12 post-oestrus. However, on day 14, control ewes responded to oxytocin with increased plasma concentrations of PGFM, whereas ewes receiving roIFN- remained unresponsive to oxytocin. These results indicate that the antiluteolytic effects of IFN- are to prevent increases in endometrial oestrogen receptor mRNA and protein and oxytocin receptor density which abrogates uterine release of prostaglandin F₂ during maternal recognition of pregnancy. IFN- may inhibit the synthesis of oestrogen receptor mRNA by a transcriptional or post-transcriptional regulatory mechanism to suppress oxytocin receptor formation during early pregnancy in ewes.

This article has been cited by other articles:

				M. M. Joyce, J. R. Burghardt, R. C. Burghardt, R. N. Hooper, F. W. Bazer, and G. A. Johnson Uterine MHC Class I Molecules and {beta}2-Microglobulin Are Regulated by Progesterone and Conceptus Interferons during Pig Pregnancy J. Immunol., August 15, 2008; 181(4): 2494 - 2505. [Abstract] [Full Text] [PDF]

				K. Ndiaye, D. H. Poole, and J. L. Pate Expression and Regulation of Functional Oxytocin Receptors in Bovine T Lymphocytes Biol Reprod, April 1, 2008; 78(4): 786 - 793. [Abstract] [Full Text] [PDF]

				S. K. Banu, J. Lee, M. C. Satterfield, T. E. Spencer, F. W. Bazer, and J. A. Arosh Molecular Cloning and Characterization of Prostaglandin (PG) Transporter in Ovine Endometrium: Role for Multiple Cell Signaling Pathways in Transport of PGF2{alpha} Endocrinology, January 1, 2008; 149(1): 219 - 231. [Abstract] [Full Text] [PDF]

				M. M. Joyce, R. C. Burghardt, R. D. Geisert, J. R. Burghardt, R. N. Hooper, J. W. Ross, M. D. Ashworth, and G. A. Johnson Pig Conceptuses Secrete Estrogen and Interferons to Differentially Regulate Uterine STAT1 in a Temporal and Cell Type-Specific Manner Endocrinology, September 1, 2007; 148(9): 4420 - 4431. [Abstract] [Full Text] [PDF]

				M. M. Joyce, J. R. Burghardt, R. C. Burghardt, R. N. Hooper, L. A. Jaeger, T. E. Spencer, F. W. Bazer, and G. A. Johnson Pig Conceptuses Increase Uterine Interferon-Regulatory Factor 1 (IRF1), but Restrict Expression to Stroma Through Estrogen-Induced IRF2 in Luminal Epithelium Biol Reprod, August 1, 2007; 77(2): 292 - 302. [Abstract] [Full Text] [PDF]

				M. C. Satterfield, F. W. Bazer, and T. E. Spencer Progesterone Regulation of Preimplantation Conceptus Growth and Galectin 15 (LGALS15) in the Ovine Uterus Biol Reprod, August 1, 2006; 75(2): 289 - 296. [Abstract] [Full Text] [PDF]

				Y. Chen, J. A. Green, E. Antoniou, A. D. Ealy, N. Mathialagan, A. M. Walker, M. P. Avalle, C. S. Rosenfeld, L. B. Hearne, and R. M. Roberts Effect of Interferon-{tau} Administration on Endometrium of Nonpregnant Ewes: A Comparison with Pregnant Ewes Endocrinology, May 1, 2006; 147(5): 2127 - 2137. [Abstract] [Full Text] [PDF]

				G. Song, F. W. Bazer, G. F. Wagner, and T. E. Spencer Stanniocalcin (STC) in the Endometrial Glands of the Ovine Uterus: Regulation by Progesterone and Placental Hormones Biol Reprod, May 1, 2006; 74(5): 913 - 922. [Abstract] [Full Text] [PDF]

				K. A. Dunlap, M. Palmarini, D. L. Adelson, and T. E. Spencer Sheep Endogenous Betaretroviruses (enJSRVs) and the Hyaluronidase 2 (HYAL2) Receptor in the Ovine Uterus and Conceptus Biol Reprod, August 1, 2005; 73(2): 271 - 279. [Abstract] [Full Text] [PDF]

				M. M. Joyce, F. J. White, R. C. Burghardt, J. J. Muniz, T. E. Spencer, F. W. Bazer, and G. A. Johnson Interferon Stimulated Gene 15 Conjugates to Endometrial Cytosolic Proteins and Is Expressed at the Uterine-Placental Interface throughout Pregnancy in Sheep Endocrinology, February 1, 2005; 146(2): 675 - 684. [Abstract] [Full Text] [PDF]

				J.G. Kim and J.L. Vallet Secreted and Placental Membrane Forms of Folate-Binding Protein Occur Sequentially During Pregnancy in Swine Biol Reprod, October 1, 2004; 71(4): 1214 - 1219. [Abstract] [Full Text] [PDF]

				T. E. Spencer, G. A. Johnson, R. C. Burghardt, and F. W. Bazer Progesterone and Placental Hormone Actions on the Uterus: Insights from Domestic Animals Biol Reprod, July 1, 2004; 71(1): 2 - 10. [Abstract] [Full Text] [PDF]

				K. A. Dunlap and F. Stormshak Nongenomic Inhibition of Oxytocin Binding by Progesterone in the Ovine Uterus Biol Reprod, January 1, 2004; 70(1): 65 - 69. [Abstract] [Full Text] [PDF]

				K. Hayashi, K. D. Carpenter, C. A. Gray, and T. E. Spencer The Activin-Follistatin System in the Neonatal Ovine Uterus Biol Reprod, September 1, 2003; 69(3): 843 - 850. [Abstract] [Full Text] [PDF]

				G. A. Johnson, R. C. Burghardt, M. M. Joyce, T. E. Spencer, F. W. Bazer, C. A. Gray, and C. Pfarrer Osteopontin Is Synthesized by Uterine Glands and a 45-kDa Cleavage Fragment Is Localized at the Uterine-Placental Interface Throughout Ovine Pregnancy Biol Reprod, July 1, 2003; 69(1): 92 - 98. [Abstract] [Full Text] [PDF]

				J. Parent, P. Chapdelaine, J. Sirois, and M. A. Fortier Expression of Microsomal Prostaglandin E Synthase in Bovine Endometrium: Coexpression with Cyclooxygenase Type 2 and Regulation by Interferon-{tau} Endocrinology, August 1, 2002; 143(8): 2936 - 2943. [Abstract] [Full Text] [PDF]

				J. E. Garlow, H. Ka, G. A. Johnson, R. C. Burghardt, L. A. Jaeger, and F. W. Bazer Analysis of Osteopontin at the Maternal-Placental Interface in Pigs Biol Reprod, March 1, 2002; 66(3): 718 - 725. [Abstract] [Full Text] [PDF]

				Y. Choi, G. A. Johnson, R. C. Burghardt, L. R. Berghman, M. M. Joyce, K. M. Taylor, M. David Stewart, F. W. Bazer, and T. E. Spencer Interferon Regulatory Factor-Two Restricts Expression of Interferon-Stimulated Genes to the Endometrial Stroma and Glandular Epithelium of the Ovine Uterus Biol Reprod, October 1, 2001; 65(4): 1038 - 1049. [Abstract] [Full Text] [PDF]

				J.-A. G. W. Fleming, Y. Choi, G. A. Johnson, T. E. Spencer, and F. W. Bazer Cloning of the Ovine Estrogen Receptor-{{alpha}} Promoter and Functional Regulation by Ovine Interferon-{{tau}} Endocrinology, July 1, 2001; 142(7): 2879 - 2887. [Abstract] [Full Text] [PDF]

				G. A. Johnson, M. D. Stewart, C. Allison Gray, Y. Choi, R. C. Burghardt, L.-Y. Yu-Lee, F. W. Bazer, and T. E. Spencer Effects of the Estrous Cycle, Pregnancy, and Interferon Tau on 2',5'-Oligoadenylate Synthetase Expression in the Ovine Uterus Biol Reprod, May 1, 2001; 64(5): 1392 - 1399. [Abstract] [Full Text]

				M. M. Mansour, M. R. Machen, B. J. Tarleton, A. A. Wiley, J. Wower, F. F. Bartol, and H. O. Goyal Expression and Molecular Characterization of Estrogen Receptor Alpha Messenger RNA in Male Reproductive Organs of Adult Goats Biol Reprod, May 1, 2001; 64(5): 1432 - 1438. [Abstract] [Full Text]

				K. M. Taylor, C. Chen, C. A. Gray, F. W. Bazer, and T. E. Spencer Expression of Messenger Ribonucleic Acids for Fibroblast Growth Factors 7 and 10, Hepatocyte Growth Factor, and Insulin-Like Growth Factors and Their Receptors in the Neonatal Ovine Uterus Biol Reprod, April 1, 2001; 64(4): 1236 - 1246. [Abstract] [Full Text]

				M. Palmarini, C. Hallwirth, D. York, C. Murgia, T. de Oliveira, T. Spencer, and H. Fan Molecular Cloning and Functional Analysis of Three Type D Endogenous Retroviruses of Sheep Reveal a Different Cell Tropism from That of the Highly Related Exogenous Jaagsiekte Sheep Retrovirus J. Virol., September 1, 2000; 74(17): 8065 - 8076. [Abstract] [Full Text]

				C. Chen, T. E. Spencer, and F. W. Bazer Fibroblast Growth Factor-10: A Stromal Mediator of Epithelial Functionin the Ovine Uterus Biol Reprod, September 1, 2000; 63(3): 959 - 966. [Abstract] [Full Text]

				H. Ka, T. E. Spencer, G. A. Johnson, and F. W. Bazer Keratinocyte Growth Factor: Expression by Endometrial Epithelia of the Porcine Uterus Biol Reprod, June 1, 2000; 62(6): 1772 - 1778. [Abstract] [Full Text]

				M. D. Stewart, G. A. Johnson, C. A. Gray, R. C. Burghardt, L. A. Schuler, M. M. Joyce, F. W. Bazer, and T. E. Spencer Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium During the Estrous Cycle and Pregnancy Biol Reprod, June 1, 2000; 62(6): 1779 - 1789. [Abstract] [Full Text]

				C. Chen, T. E. Spencer, and F. W. Bazer Expression of Hepatocyte Growth Factor and Its Receptor c-metin the Ovine Uterus Biol Reprod, June 1, 2000; 62(6): 1844 - 1850. [Abstract] [Full Text]

				G. A. Johnson, T. E. Spencer, R. C. Burghardt, K. M. Taylor, C. A. Gray, and F. W. Bazer Progesterone Modulation of Osteopontin Gene Expression in the Ovine Uterus Biol Reprod, May 1, 2000; 62(5): 1315 - 1321. [Abstract] [Full Text]

				K. M. Taylor, C. A. Gray, M. M. Joyce, M. D. Stewart, F. W. Bazer, and T. E. Spencer Neonatal Ovine Uterine Development Involves Alterations in Expression of Receptors for Estrogen, Progesterone, and Prolactin Biol Reprod, April 1, 2000; 63(4): 1192 - 1204. [Abstract] [Full Text]

				G. A. Johnson, T. E. Spencer, R. C. Burghardt, M. M. Joyce, and F. W. Bazer Interferon-Tau and Progesterone Regulate Ubiquitin Cross-Reactive Protein Expression in the Ovine Uterus Biol Reprod, March 1, 2000; 62(3): 622 - 627. [Abstract] [Full Text]

				T. E. Spencer, A. Gray, G. A. Johnson, K. M. Taylor, A. Gertler, E. Gootwine, T. L. Ott, and F. W. Bazer Effects of Recombinant Ovine Interferon Tau, Placental Lactogen, and Growth Hormone on the Ovine Uterus Biol Reprod, December 1, 1999; 61(6): 1409 - 1418. [Abstract] [Full Text]

				G. A. Johnson, T. E. Spencer, R. C. Burghardt, and F. W. Bazer Ovine Osteopontin: I. Cloning and Expression of Messenger Ribonucleic Acid in the Uterus During the Periimplantation Period Biol Reprod, October 1, 1999; 61(4): 884 - 891. [Abstract] [Full Text]

				T. E. Spencer, A. G. Stagg, M. M. Joyce, G. Jenster, C. G. Wood, F. W. Bazer, A. A. Wiley, and F. F. Bartol Discovery and Characterization of Endometrial Epithelial Messenger Ribonucleic Acids Using the Ovine Uterine Gland Knockout Model Endocrinology, September 1, 1999; 140(9): 4070 - 4080. [Abstract] [Full Text]

				T. E. Spencer, A. G. Stagg, T. L. Ott, G. A. Johnson, W. S. Ramsey, and F. W. Bazer Differential Effects of Intrauterine and Subcutaneous Administration of Recombinant Ovine Interferon Tau on the Endometrium of Cyclic Ewes Biol Reprod, August 1, 1999; 61(2): 464 - 470. [Abstract] [Full Text]

				B. J. Tarleton, A. A. Wiley, and F. F. Bartol Endometrial Development and Adenogenesis in the Neonatal Pig: Effects of Estradiol Valerate and the Antiestrogen ICI 182,780 Biol Reprod, July 1, 1999; 61(1): 253 - 263. [Abstract] [Full Text]

				G. A. Johnson, T. E. Spencer, T. R. Hansen, K. J. Austin, R. C. Burghardt, and F. W. Bazer Expression of the Interferon Tau Inducible Ubiquitin Cross-Reactive Protein in the Ovine Uterus Biol Reprod, July 1, 1999; 61(1): 312 - 318. [Abstract] [Full Text]

				J. A. McCracken, E. E. Custer, and J. C. Lamsa Luteolysis: A Neuroendocrine-Mediated Event Physiol Rev, April 1, 1999; 79(2): 263 - 323. [Abstract] [Full Text] [PDF]

				C.-S. Han, N. Mathialagan, S. W. Klemann, and R. M. Roberts Molecular Cloning of Ovine and Bovine Type I Interferon Receptor Subunits from Uteri, and Endometrial Expression of Messenger Ribonucleic Acid for Ovine Receptors During the Estrous Cycle and Pregnancy Endocrinology, November 1, 1997; 138(11): 4757 - 4767. [Abstract] [Full Text] [PDF]