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We have investigated the expression of oestrogen receptor (ER) mRNA in Ficoll-separated tonsillar cells and the changes that occur with the addition of oestradiol (OE₂) both in the presence and the absence of the T cell mitogen phytohaemagglutinin (PHA). The amounts of ER mRNA and β-actin mRNA in the samples were determined by slot blotting and hybridization and quantified by densitometry. The levels of ER mRNA were normalized against the β-actin mRNA content. In the presence of OE₂ (7x10^–8M) after a 10-h culture there was a significant decrease (P<0·05) to about 66% of the control (0-h culture) ER mRNA levels. Stimulating the cultures with PHA (1 µg/ml, without the presence of OE₂, had no effect on the expression of ER mRNA. However, when OE₂ was present in a 10-h culture of PHA-stimulated cells, the ER mRNA level was significantly decreased (P<0·05) to about 60% of control levels. In 24-h cultures, the presence of OE₂ and/or PHA had no effect. When separated T cell preparations from the tonsils were used, no significant effects of OE₂ were seen in either the 10-h or 24-h cultures. In conclusion, OE₂ downregulates the ER mRNA content in a tonsillar mononuclear cell system in vitro as it does in many primary oestrogen target cells.

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