HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Haraguchi, K Articles by Onaya, T Search for Related Content PubMed PubMed Citation Articles by Haraguchi, K Articles by Onaya, T

To understand the functional significance of the carboxy-terminal half of the intracellular region of the human TSH receptor (hTSHR), a mutant hTSHR lacking amino acids from the carboxyterminal to His726 was constructed.

Wild type hTSHR cDNA and truncated hTSHR cDNA were subcloned into a eukaryotic expression vector, pRc/CMV, and transfected into Chinese hamster ovary cells to obtain cell lines which stably expressed hTSHRs at high levels. This allowed us to observe highly efficient coupling of hTSHR and adenylyl cyclase as well as desensitization and internalization of hTSHR.

Despite the differences in potential phosphorylation sites and internalization signals, dose-dependent stimulation of adenylyl cyclase by TSH, TSH-dependent desensitization and the rate of hTSHR internalization were similar for wild type and truncated hTSHRs. We conclude that the carboxy-terminal half of the intracellular region of hTSHR does not have a major functional role in TSH-dependent signal transduction.

This article has been cited by other articles:

				K. A Heemstra, W. M van der Deure, R. P Peeters, N. A Hamdy, M. P Stokkel, E. P Corssmit, J. A Romijn, T. J Visser, and J. W Smit Thyroid hormone independent associations between serum TSH levels and indicators of bone turnover in cured patients with differentiated thyroid carcinoma. Eur. J. Endocrinol., July 1, 2008; 159(1): 69 - 76. [Abstract] [Full Text] [PDF]

				L. E. Ericson and M. Nilsson Deactivation of TSH receptor signaling in filter-cultured pig thyroid epithelial cells Am J Physiol Endocrinol Metab, April 1, 2000; 278(4): E611 - E619. [Abstract] [Full Text] [PDF]

				C. Baratti-Elbaz, N. Ghinea, O. Lahuna, H. Loosfelt, C. Pichon, and E. Milgrom Internalization and Recycling Pathways of the Thyrotropin Receptor Mol. Endocrinol., October 1, 1999; 13(10): 1751 - 1765. [Abstract] [Full Text]

				Y. Nagayama, K. Tanaka, T. Hara, H. Namba, S. Yamashita, K. Taniyama, and M. Niwa Involvement of G Protein-coupled Receptor Kinase 5 in Homologous Desensitization of the Thyrotropin Receptor J. Biol. Chem., April 26, 1996; 271(17): 10143 - 10148. [Abstract] [Full Text] [PDF]