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We studied the hormonal secretion of a human mixed follicular and medullary carcinoma. Thyroglobulin (Tg) secretion, especially by large cells and sometimes by small ones, was visualized with immunoenzymatic staining. Calcitonin (CT) was produced by small spindle-shaped cells. Moreover, immunofluorescence double staining performed on the resected thyroid tissue showed the secretion of both Tg and CT in a small number of cells. The cells lost their hormonal secretion after 2 months of culture. Hormonal secretion was modulated by different additives in the medium. Tg secretion was induced when TSH was added to the culture medium; the maximal effect was produced with the addition of 1 mU TSH/ml and 1 µM cortisol, which potentiated the effect of TSH on Tg production. A durable Tg secretion was obtained by embedding the cells in Engelbretch—Hohn—Swarn (EHS) tumour matrix. The CT production was reinduced by the addition of 4 mM Ca²⁺, 1 µM glucagon and 1 µM cortisol to the culture medium. These findings show that different cells are found in a mixed follicular and medullary carcinoma, some of which can secrete both CT and Tg. They can remain differentiated for a long period after being embedded in EHS tumour matrix with Ca2⁺ and hormonal components.

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				M. Volante, M. Papotti, J. Roth, P. Saremaslani, E. J. M. Speel, R. V. Lloyd, J. A. Carney, P. U. Heitz, G. Bussolati, and P. Komminoth Mixed Medullary-Follicular Thyroid Carcinoma : Molecular Evidence for a Dual Origin of Tumor Components Am. J. Pathol., November 1, 1999; 155(5): 1499 - 1509. [Abstract] [Full Text] [PDF]