| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
The human oestrogen receptor (hER) gene has recently been shown to be transcribed from two different promoters. In order to study the hER gene promoter region we initially isolated and sequenced 3·3 kb of the 5' region of the hER gene. DNase I hypersensitivity experiments with nuclei from the ER-expressing cell line MCF-7 mapped three different hypersensitive sites, located in the vicinity of the two hER promoters previously identified. However, in another ER-positive cell line, ZR-75-1, DNase I hypersensitivity was observed only in the region of the downstream promoter, suggesting differences between the two cell lines in promoter usage. Polymerase chain reaction-mediated detection of mRNAs transcribed from the two different promoters demonstrated transcripts from the downstream promoter in both the cell lines studied. However, mRNAs transcribed from the upstream promoter could only be detected in MCF-7 cells. Thus, the pattern of DNase I hypersensitivity correlates with the transcriptional activity of the two promoters in the hER gene. Based on these data, we suggest that differential promoter usage might be a novel level of regulation of the hER gene.
This article has been cited by other articles:
|
|
 |
|
  L. Gennari, D. Merlotti, V. De Paola, A. Calabro, L. Becherini, G. Martini, and R. Nuti Estrogen Receptor Gene Polymorphisms and the Genetics of Osteoporosis: A HuGE Review Am. J. Epidemiol., February 15, 2005; 161(4): 307 - 320. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kos, G. Reid, S. Denger, and F. Gannon Minireview: Genomic Organization of the Human ER{alpha} Gene Promoter Region Mol. Endocrinol., December 1, 2001; 15(12): 2057 - 2063. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Yoshida, H. Eguchi, K. Nakachi, K. Tanimoto, Y. Higashi, K. Suemasu, Y. Iino, Y. Morishita, and S.-i. Hayashi Distinct mechanisms of loss of estrogen receptor {{alpha}} gene expression in human breast cancer: methylation of the gene and alteration of trans-acting factors Carcinogenesis, December 1, 2000; 21(12): 2193 - 2201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Becherini, L. Gennari, L. Masi, R. Mansani, F. Massart, A. Morelli, A. Falchetti, S. Gonnelli, G. Fiorelli, A. Tanini, et al. Evidence of a linkage disequilibrium between polymorphisms in the human estrogen receptor {alpha} gene and their relationship to bone mass variation in postmenopausal Italian women Hum. Mol. Genet., August 12, 2000; 9(13): 2043 - 2050. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Donaghue, B. R. Westley, and F. E. B. May Selective Promoter Usage of the Human Estrogen Receptor-{alpha} Gene and Its Regulation by Estrogen Mol. Endocrinol., November 1, 1999; 13(11): 1934 - 1950. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. L. O’Brien, K. Park, Y. In, and O.-K. Park-Sarge Characterization of Estrogen Receptor-{beta} (ER{beta}) Messenger Ribonucleic Acid and Protein Expression in Rat Granulosa Cells Endocrinology, October 1, 1999; 140(10): 4530 - 4541. [Abstract] [Full Text]
|
|
|
|
|
|
I. Treilleux, N. Peloux, M. Brown, and A. Sergeant Human Estrogen Receptor (ER) Gene Promoter-P1: Estradiol-Independent Activity and Estradiol Inducibility in ER+ and ER- Cells Mol. Endocrinol., August 1, 1997; 11(9): 1319 - 1331. [Abstract] [Full Text]
|
|
|
|
|
|
G. G. J. M. Kuiper, B. Carlsson, K. Grandien, E. Enmark, J. Haggblad, S. Nilsson, and J.-A. Gustafsson Comparison of the Ligand Binding Specificity and Transcript Tissue Distribution of Estrogen Receptors {alpha} and {beta} Endocrinology, March 1, 1997; 138(3): 863 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. R. Schuur, L. A. McPherson, G. P. Yang, and R. J. Weigel Genomic Structure of the Promoters of the Human Estrogen Receptor-alpha Gene Demonstrate Changes in Chromatin Structure Induced by AP2gamma J. Biol. Chem., April 27, 2001; 276(18): 15519 - 15526. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
