JME
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Journal of Molecular Endocrinology (1993) 10, 207-214    DOI: 10.1677/jme.0.0100207
© 1993 Society for Endocrinology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Señarís, R M
Right arrow Articles by Diéguez, C
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Señarís, R M
Right arrow Articles by Diéguez, C

Effects of free fatty acids on somatostatin secretion, content and mRNA levels in cortical and hypothalamic fetal rat neurones in monolayer culture

 R M Señarís, M D Lewis, F Lago, F Domínguez, M F Scanlon and C Diéguez

The effects of free fatty acids on somatostatin secretion, content and mRNA levels in fetal rat hypothalamic and cortical cell cultures were investigated. Somatostatin secretion and content were quantified by radioimmunoassay. Somatostatin mRNA levels were measured by Northern blot hybridization using a cRNA probe. Treatment with either caprylic acid (5x10-3M) or oleic acid (5x 10-5 M) for 90 min inhibited basal somatostatin secretion in both hypothalamic and cortical cell cultures. In addition, the increase in somatostatin secretion induced by incubation with veratridine (10-4M) or carbachol (10-4M) for 90 min was significantly reduced by the addition of caprylic acid, but somatostatin release stimulated by 5·6x10-2M KCl was not affected. Furthermore, treatment with these free fatty acids for 90 min markedly decreased somatostatin mRNA levels in both types of neurone culture. These inhibitory effects were transient, being observed after 90 min, but not after 5 h. These results support the probability that there is a role for free fatty acids in the regulation of somatostatin mRNA levels and somatostatin secretion in both hypothalamic and cortical cell cultures.




This article has been cited by other articles:


Home page
 Eur J EndocrinolHome page
F. Lanfranco, L. Gianotti, A. Picu, R. Giordano, G. A. Daga, V. Mondelli, G. Malfi, S. Fassino, E. Ghigo, and E. Arvat
Effects of free fatty acids on ACTH and cortisol secretion in anorexia nervosa.
Eur. J. Endocrinol., May 1, 2006; 154(5): 731 - 738.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
F. Lanfranco, R. Giordano, M. Pellegrino, L. Gianotti, J. Ramunni, A. Picu, M. Baldi, E. Ghigo, and E. Arvat
Free Fatty Acids Exert an Inhibitory Effect on Adrenocorticotropin and Cortisol Secretion in Humans
J. Clin. Endocrinol. Metab., March 1, 2004; 89(3): 1385 - 1390.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Ciampelli, G. Muzj, F. Leoni, D. Romualdi, C. Belosi, R. M. Cento, and A. Lanzone
Metabolic and Endocrine Consequences of Acute Suppression of FFAs by Acipimox in Polycystic Ovary Syndrome
J. Clin. Endocrinol. Metab., November 1, 2001; 86(11): 5324 - 5329.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
E. E. Muller, V. Locatelli, and D. Cocchi
Neuroendocrine Control of Growth Hormone Secretion
Physiol Rev, April 1, 1999; 79(2): 511 - 607.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Quintela, R. M. SenarIs, and C. Dieguez
Transforming Growth Factor-{beta}s Inhibit Somatostatin Messenger Ribonucleic Acid Levels and Somatostatin Secretion in Hypothalamic Cells in Culture
Endocrinology, October 1, 1997; 138(10): 4401 - 4409.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1993 by the Society for Endocrinology.